What can be the presumable reason for the lower activity of cellulase at 5mM concentration of metal ions than at 2 &10mM concentration ?

I have purified enzymes cellulase and beta-glucosidase and performed SDS-PAGE. Therefore, I have characterized with metal ions at 2mM, 5mM and 10mM but at 5mM concentration lower activity than at...

25 March 2020 8,056 3 View

How are digestive enzymes for human consumption commercially produced?

Hello, Many protein shake powder mixes contain digestive enzymes such as protease, lipase, and cellulase. However, the products do not detail from which organism the proteins are produced or...

21 March 2020 5,048 3 View

How to convert the Fillter paper unit to G?

I need to perform enzymatic saccharification process for that i need to add cellulase enzyme. In the Ref paper everyone put as FPU. i dont know to convert FPU into mg. can anyone help with this?

23 February 2020 8,297 2 View

Suitable sample types for assaying cellulase activity?

Hello, I am trying to set up an enzymatic assay to determine the activity of cellulase. I would like to know what sample types would be suitable to perform the same? I have read certain papers...

01 February 2020 1,105 3 View

How to convert the Fillter paper unit to G?

I need to perform enzymatic saccharification process for that i need to add cellulase enzyme. In the Ref paper everyone put as FPU. i dont know to convert FPU into mg. can anyone help with this?

01 February 2020 8,283 2 View

How to create a standard curve of enzyme concentration and relative absorbance?

Hello, during an experiment I found myself in needing a standard curve of a kit for enzyme activity screening. The kit has a certain amount of substrate (cellulose) and I prepared different...

01 February 2020 2,552 3 View

How to measure the protein loading of enzyme immobilization?

I'm trying to immobilize cellulase on Fe3O4 magnetic nanoparticles coated with Tannic acid, with glutaraldehyde as cross-linker. After the immobilization process, I washed nanoparticles four times...

01 February 2020 5,830 5 View

Besides the issues of buffer pH in relation to pI, what other factors could make an enzyme protein not to bind to a cation or anion exchange resin?

The cellulase enzymes I am purifying did not bind to DEAE cellulose and CM sapharose columns using an acetate buffer, pH 5.0. The pI of the enzyme protein is 7.0. Please could someone help me with...

31 December 2019 4,489 13 View

Cellulase enzyme loaded based on hydrolysis activity and protein basis, what does protein basis mean?

10 November 2019 9,320 3 View

How to calculate the dosage of enzymes in wheat flour?

I have recently started working on improvement of wheat flour for baking. I have come across a research which involved the usage of xylanase, cellulase, beta-glucanase and carbohydrases for...

10 November 2019 3,608 1 View