I am performing wastewater treatment thought different microorganisms such as microalgae and afterwards determining the lipid content in the biomass though Bligh and dyer method. Due to the COVID restriction, we are allowed to have limited time in the laboratory and therefore, a lot of samples should be stored and tested later.
I have to store my sample for cell counting and lipid determination?
For cell counting, Can I store my sample in ethanol to persevere it and count it later?
(0.3 ml sample and 0.7 ml ethanol)
For lipid: Can I store my liquid sample at -20C. and perform the lipid determination by Bligh and Dyer method later?
For cell counting you can just add 1-2 drops of lugol solution to a 10 ml sample and store it.
For lipid determination, I recommend you to store your Microalgae pellet in the own extraction solvent with an antioxidant (BHT is commonly used) and inert (N gas) atmosphere in the freezer (-20-40ºC is OK)
Jose Pedro Canavate : Thank you for your response.
Do you have any references for this? Would be happy to read about it in detail.
For cell count, I not sure about adding ethanol, because I never store the sample for cell count. in my case, I take the picture of the cell count on the hemocytometer slide and save it to my laptop, to be count later.
For lipid better use the BHT before deep freeze.
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