Im a biochemist so this really isn't my wheelhouse but can anyone tell me the typically LLoD and LLoQ of NGS like RNA-seq and DNA-seq in molar? Most articles provide them in percentage. With references would be even better! Thanks!
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...
31 July 2024 2,033 1 View
Am trying to develop a lesson/lab to determine and compare antioxidant properties of soybeans of various colors. Preference would be low tech and low cost. Any assistance is greatly appreciated.
25 July 2024 7,498 2 View
In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....
24 July 2024 8,076 3 View
Their is new research published in the biological control journal, and I am one of the authors. I want to add it to my account at researchgare. How can I do this?
21 July 2024 7,545 0 View
I have read that it is better to use other research methods alongside document analysis for triangulation, although it can also be used as a stand-alone method.
18 July 2024 4,218 0 View
I am conducting a study on Effectiveness of e-content on academic achievement of secondary school science student.
17 July 2024 5,633 4 View
I am simulating interlocking / dry stacked masonry in Abaqus. I am using a friction coefficient of 0.6 for my model as it is stated in the previous research as well as in the base paper. The...
16 July 2024 5,047 0 View
im studing gaussian 16 with reading paper about I-131 Metaiodobenzylguanidine in the paper "In a similar vein, nuclear magnetic resonance shielding values were investigated using the widely...
16 July 2024 6,040 4 View
I am doing a systematic review, and I am measuring risk of bias with RoB2 for RCT, and ROBINS-I for non RCT. My questions is, for single arm studies, can I use ROBINS-I? I am not sure how to...
14 July 2024 3,570 5 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?
06 August 2024 980 5 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View