Hello everyone, have anyone an experience about Transfection of Human U87 GBM cell line with PEI?

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Hello everyone, have anyone an experience about ligation with one digested vector(salI) with double digestion insert(SalI XhoI) that ligate together?

My vector is 7000 bps, and my insert is 1300 bps

28 May 2024 831 4 View

Are You an Expert in Plant Abiotic Stress Response? Interested in Collaborating on Paper Writing?

I have been recently working on abiotic stresses (drought, high temperature, salinity and cold) responses in wheat (Triticum aestivum) using meta-analysis of transcriptomics (microarray) data. The...

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How can I optimize parameters of cyclic voltammetry to observe oxidation peaks?

I am investigating the redox behavior of Diene compounds by cyclic voltammetry in anaerobic environment. My electrodes include carbon glassy electrode as working electrode, platinum as counter and...

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How can we explain the expression changes between a total and phosphorylated form of a protein?

I am analyzing the expression of a receptor tyrosine kinases protein and its phosphorylated form via Western blots. The phosphorylated form has decreased significantly, and the total remains...

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Why there is no endogenous expression of my protein?

I tried to detect the endogenous expression of my protein of interest, but I can't. However, at the same time, I can verify the overexpression of the protein with different antibodies. I tried...

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DSP (cross-linking reagent) for suspension cells?

Hello, Has anyone ever used DSP ( a cross-linking reagent) for suspension cells before preparing cell lysate for performing co-Immunoprecipitation?

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Is there a solution to stain the bacteria and quench the dye and use another dye like DAPI to stain the cell?

In my project, I am trying to show Staphylococcus aureus in endothelial cells with fluorescent dyes. But when I used calcein AM, due to the presence of esterase enzyme in the bacteria and the...

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How to improve the concentration of input samples during immunoprecipitation?

Hello, I am doing co-immunoprecipitation to study the interaction of my protein of interest. The protein concentration after performing the Bradford assay for input samples is deficient. Does...

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How can I export voxel based file or tetra grid mesh from Avizo ?

I want to provide a voxel-based file or tetra grid mesh geometry for analysis in solvers like Abaqus. always generating tetra mesh face with different errors like the following figure. Does...

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How can I solve this error in Abaqus?

I exported the model from 3 matics to Abaqus for analysis but was faced with this error : 6766 elements have missing property definitions. The elements have been identified in element set...

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Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

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Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

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Is there any way to quantify bacterial and fungal cells in their mixed culture?

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How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

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Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

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How to isolate lymphocytes from mouse spleen?

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How to Add Missing Water Molecules in Protein-Membrane Simulations?

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Does long-term culture of murine Monocytes result in macrophage differentiation?

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To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

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03 August 2024 3,851 1 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

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Kamran Hosseini

Yes

Shirin Shahsavari

Hello, Thanks a lot for your answer, Can I ask what kinds of Buffer you have used? and how was efficiency of your transfection?

Please give me your email to send guideline for you

Hello,

my email: [email protected] ,

Thanks a lot for your attention,

Best regards