Hello everyone,

I’m planning an experiment to measure the expression of beta defensins in HeLa cells stimulated with Candida albicans using indirect immunofluorescence.

I’m wondering if it’s necessary to use monensin and brefeldin A to block protein secretion for better detection, or if the defensins can be adequately detected without these inhibitors.

Has anyone worked with similar conditions and could provide insights on whether these inhibitors are required for accurate detection? Additionally, if anyone has a protocol or specific conditions for using these inhibitors, I would greatly appreciate it if you could share them.

Thank you for your input!