Has anybody ever tried to perform antigen retrieval on frozen tissue sections by incubation in sucrose? We tried that in case of murine iNOS in the spinal cord and it works pretty nice. However, I found just one note on this in the literature:
Article Shi, S.R., Cote, R.J. & Taylor, C.R. Antigen retrieval immun...
- section "Non-heating Method"(Nota bene, I do NOT ask for sucrose incubation in order to cryoprotect the samples.)
Could you provide more details on what you do? Was the tissue chemically fixed before retrieval?
Vivica Grotelueschen: We perfused the mouse with freshly prepared formaldehyde, isolated the spinal cord and post-fixed in formadehyde overnight. Then we cryo-cut the sample and tried different antigen retrieval methods (citrate, SDS, sucrose).
I have discovered a new method for detection the mitotic activity in cancer ( The iNOS has relation with tumor) , I didnt use the antigen retrieval .
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