Does anyone have experience with AML12 cells and knows how to push the cells to synthesize lipid droplets?
I am using DMEM/F-12 medium, 15% FBS and ITS-G(1:100) and still there is just a few lipid droplets in the flask.
Thank u
Sorry, dont have
Havent worked with AML 12 cells but lipid droplets may be increased by treating the cells with oleic acid complexed with BSA. By google you will find many protocols for this.
200 µM OA complexed with BSA in growth medium overnight, there would be many LDs.
Thank you :)
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