Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

What are the conflicts between EIA and environmental engineers and lawyers?

Environmental Impact Assessment (EIA) does not live up to the expectation of environmental lawyers and environmental engineers. In your own opinion, how can the EIA process therefore be made more...

03 February 2021 1,329 6 View

How to prepare secondary antibody from lyophilized antibody?

So I'm new to lab research and learning Western Blots these days. I ordered my secondary antibody and it arrived in a powdered form. Here is the...

27 January 2021 5,402 3 View

Colorimteric and enzymatic method ?

First of all, thank you for your consideration about this question. I've been working on development of in-vitro diagnostic medical device as research engineer since 2013. Especially, my major is...

14 January 2021 3,885 4 View

Which syringe filters (e.g., PTFE, MCE, PVDF, CA, PES, etc.) are the best to filter TBS/PBS buffer (pH 7.5-8.5) and glycine buffer (pH 2-3) solutions?

I intend to purify antibodies from serum using the affinity procedure, and I need to filter the binding (TBS/PBS) and elution (Glycine) buffers before applying them to my solution.

06 January 2021 5,769 3 View

ELISPOT normalization with varying cell numbers?

Hello everyone, due to a calculation error in the spreadsheet, the splenocytes I used were wrongly diluted. As a result, the number of cells per well are identical for each mouse, but differ...

15 December 2020 9,344 1 View

Greiner Bio or Corning Costar plates for ELISA?

09 December 2020 237 2 View

What is optimal buffer for saliva immunoglobulins testing with lateral flow immunoassay?

Hi to everyone! Does anybody know optimal buffer for detecting HIV-IgG with lateral flow immunoassay using saliva as a specimen type? I think that i need a buffer for LFIA becouse saliva too...

16 November 2020 1,520 3 View

Can primary antibodies bind to analyte coupled magnetic beads directly if the coupling was unsuccessful ?

I am working with multiplex immunoassay. As I am new into multiplexing I am facing some problem. I have coupled Protein X to carboxylated magnetic beads (BioRad) using EDC-NHS. Now, while...

08 November 2020 9,911 7 View

Which organic solvent should I choose to make a cortisol stock solution for immunoassay?

Hi everyone, I plan to make my own cortisol standards for immunoassay. Hydrocortisone (cortisol) is soluble in organic solvents, such as ethanol, methanol, DMF etc., but I'm not sure which one...

01 September 2020 3,442 4 View