Why Negative peak eluted in HPLC analysis?

I'facing problem while doing HPLC analysis due to negative peak was eluted which was corresponding to the principle peak RT. I tried to remove, with different solvents, but no use. i want to know...

26 November 2020 1,960 5 View

Is there any list available of GRAS (generally recognized as safe) microorganisms with proper references?

Is there any list available of GRAS (generally recognized as safe) microorganisms with proper references?

02 March 2020 1,011 2 View

Which stain is used to see the deposition of oil or oil glands in anatomy section of leaf(transverse section) in cymbopogon?

Cymbopogon is an aromatic grass which produces essential oils as a secondary metabolite so to see the deposition of oil in leaves transverse section of intra and inter species can you suggest me...

28 August 2019 9,357 2 View

What is the difference between lithium ion and lithium polymer battery?

Lithium ion and lithium polymer battery charging and discharging profile was found to be same. Then what is the major difference between both battery?

07 July 2019 9,301 4 View

Homing Receptors for tumor infiltrating T cells?

I am researching how different culture and expansion conditions contribute to a T cell's infiltration of a solid tumor. Was wondering what the best surface / intracellular receptors or ligands are...

26 June 2019 5,072 0 View

What are my THP-1 cells contaminated with?

Hello everyone, I recently thawed THP-1 cells, which were frozen at passage 12 in 2016. I got the cells back to culture in RPMI + 10% FCS + 1% HEPES + 1% PenStrep. The cells seemed to recover...

19 June 2019 7,802 6 View

Temperature rise in unloaded solar cell?

Im doing research on temperature rise in solar cell during open circuit condition or partially loaded. Im sure that when solar cell is in open circuit the energy generated will be transferred as...

04 January 2019 9,904 9 View

Can anyone refer me list of terpene and terpenoid with their Rf values?

I am doing tlc of cymbopogon martini leaves extract (methanol chloroform and Ethyl acetate extract ) can anyone suggest me papers for identifying compounds (terpenoid compounds) with their rf values

17 December 2018 2,817 3 View

Which is the most appropriate tool for testing acaricide resistance of field ticks by molecular methods?

within the PCR which test is most appropriate? For example Allele specific PCR?

16 May 2018 5,773 1 View

Is there a way to check for tranfection and retrovirus production?

04 October 2017 6,478 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

What are the conflicts between EIA and environmental engineers and lawyers?

Environmental Impact Assessment (EIA) does not live up to the expectation of environmental lawyers and environmental engineers. In your own opinion, how can the EIA process therefore be made more...

03 February 2021 1,329 6 View

How to prepare secondary antibody from lyophilized antibody?

So I'm new to lab research and learning Western Blots these days. I ordered my secondary antibody and it arrived in a powdered form. Here is the...

27 January 2021 5,402 3 View

Colorimteric and enzymatic method ?

First of all, thank you for your consideration about this question. I've been working on development of in-vitro diagnostic medical device as research engineer since 2013. Especially, my major is...

14 January 2021 3,885 4 View

Which syringe filters (e.g., PTFE, MCE, PVDF, CA, PES, etc.) are the best to filter TBS/PBS buffer (pH 7.5-8.5) and glycine buffer (pH 2-3) solutions?

I intend to purify antibodies from serum using the affinity procedure, and I need to filter the binding (TBS/PBS) and elution (Glycine) buffers before applying them to my solution.

06 January 2021 5,769 3 View

ELISPOT normalization with varying cell numbers?

Hello everyone, due to a calculation error in the spreadsheet, the splenocytes I used were wrongly diluted. As a result, the number of cells per well are identical for each mouse, but differ...

15 December 2020 9,344 1 View

Greiner Bio or Corning Costar plates for ELISA?

09 December 2020 237 2 View

What is optimal buffer for saliva immunoglobulins testing with lateral flow immunoassay?

Hi to everyone! Does anybody know optimal buffer for detecting HIV-IgG with lateral flow immunoassay using saliva as a specimen type? I think that i need a buffer for LFIA becouse saliva too...

16 November 2020 1,520 3 View

Can primary antibodies bind to analyte coupled magnetic beads directly if the coupling was unsuccessful ?

I am working with multiplex immunoassay. As I am new into multiplexing I am facing some problem. I have coupled Protein X to carboxylated magnetic beads (BioRad) using EDC-NHS. Now, while...

08 November 2020 9,911 7 View

Which organic solvent should I choose to make a cortisol stock solution for immunoassay?

Hi everyone, I plan to make my own cortisol standards for immunoassay. Hydrocortisone (cortisol) is soluble in organic solvents, such as ethanol, methanol, DMF etc., but I'm not sure which one...

01 September 2020 3,442 4 View