Best purification method for recombinant protein in BL21?

Hi I need to purify a recombinant protein (IL6 21kDa). It's been produced in E.coli BL21 with a T7 expression vector, but it does not have a tag as yet. I was thinking of adding a his-tag and...

09 December 2020 2,200 8 View

Recombinant Interleukin 6 western blot, non specific bands?

Hi I've been trying to produce some recombinant human Interleukin 6. I sub-cloned the gene for human IL6 into a Promega T7 Flexi vector (native gene no tag yet) and confirmed sequencing and...

09 December 2020 2,340 4 View

Can anyone suggest me a solvent ratio to increase the gap between these two spots?

After column chromatography I've got these two spots together. And tried a number of solvents ratio to increase the gap between these two spots for further column chromatography but failed.

10 October 2020 1,367 4 View

What is the appropriate length of packed silica for column chromatography?

In the case of column chromatography of plant extract, which is the appropriate length of packed silica of a one-inch diameter and 18 inches long column, and what speed should be used for solvent...

29 September 2020 4,818 8 View

Should I use glass vials for mouse organ preservation for short term or plastic vials are good enough for short term storage?

I have noticed many researchers use both glass and plastic vials for mouse organ preservation. Which one is better in case of short term storage?

19 July 2020 4,928 4 View

Why am I getting overestimated DNA concentration by A260 after phenol chloroform extraction?

I performed a standard PCIA extraction (2x PCIA, 1X chloroform) on my PCR samples to clean them up for use as an enzyme substrate. By A260, (nanodrop) I later found I have overestimated the [DNA]...

25 March 2020 1,007 6 View

How to set a target frequency goal in CST Studio 2019?

Hello, I am new to CST having only started working with this software this year. I am a student, for a piece of work I am currently doing I have been provided with models of CSRR's and need to...

14 March 2020 7,084 3 View

For immunoflorescence stains on oral mucosa tissue, how do you differentiate cross-section skeletal muscle from adipose tissue that both autofloresce?

I am performing macrophage staining in the oral mucosa using an IFA protocol. There are oval-shaped structures on my scans that have background stain/autoflorescence. After doing some research, I...

27 January 2020 9,940 1 View

I am a colege student in a institute teknology, now i begin to make a research. And i confuse, iam not have idea. Please some one help me..?

Please give Me idea to begin my reserch in electricitian. Thankyou very much

03 January 2020 8,855 2 View

Hi Maryanne, where do you stand on including social competition under the term female intrasexual competition as well as competition for mates?

There is always a lot of confusion and negative reaction when I discuss female intrasexual competition with lay people, especially women. Many feel the "female" is unnecessary and sexist or that...

19 January 2019 1,844 1 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

The procedure compute?

02 March 2021 5,204 3 View