It depends on what type of input image you are working with. Whole slide images will require a different approach to microscope images due to the way they are compressed. For whole slide images I would look at - https://imagej.net/SlideJ
If you're just starting out I would save small snapshots of the whole slide as tiff files and play around with opening them in Fiji and segmenting out your areas of interest using colour thresholds and then measuring the features using Measure or Analyse Particles.
This will help you.
https://www.researchgate.net/post/Does_anyone_knows_how_to_use_Fiji_ImageJ_software_to_stitch_histological_slices
It depends on what you would like to measure. To separate H and E dyes to quantify them separately you can use colour deconvolution: https://imagej.net/Colour_Deconvolution
To estimate e.g. cells number - you can use one of the plugins to do this after deconvolution/thresholding (but for cell counting I will strongly recommend stereology), to measure areas - you can outline your ROI by hand and measure it using Fiji, you can also use threshold and binarize the picture to measure its features etc, etc, there are many possibilities, depending on what should be measured and how the tissue looks like.
Firstly haematoxylin and eosin stain often abbreviated as:(H&E stain or HE stain) is one of the principal tissue stains used in histology .it is most widely used stain in medical diagnosis and often the gold standard e.g when a pathologist is working on a biopsy suspect with cancer.histological section is likely to be stained with H& E .H&E is the combination of two histological stains,haematoxylin and eosin.The haematoxylin stains the nuclei blue ,and eosin stains the extracellular matrix and cytoplasm. Pink,with other structures taking on different shades,lines and combinations on these colours.the stains shows the general layout and distribution of cells.
In the case of H$E histology image for tissue using fiji.
Fiji stain in histology is just talking about the conventional method and autoflouresence images of unlabelled tissue sections into images that are equivalent to the bright field images of histologically stained version of the samples.
And also virtual staining method and standard histology staining using microscopic images of human tissues sections of salivary gland ,thyroid ,kidney ,liver and lung ,and involving different types of stain ,showed no major discordances.the virtual staining method bypasses the typically labour intensive and cost be used as a blueprint for the virtual staining of tissue images acquired with other label free imaging modalities.
H& E are important stains used to differentiate the all tissue by stain the nucleus & the cytoplasm of the cell, also used to determinate the normal & abnormal tissues
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To quantify for H$E histology image for tissue using Fiji .
You can use a conventional method and autoflouresence images of unlabelled tissue sections into images that are equivalent to the bright field images of histologically stained versions of the samples.
And also virtual staining method and standard histological staining using microscopic images of human tissue section such as lungs,kidney,liver and thyroid gland ,and involving different types stain,show no major discordances.
The virtual staining method bypasses the typically labour intensive and costly histological staining procedures and cost be used as a blueprint for the virtual staining of tissue images acquired with other label free imaging modalities.
this link help you. https://www.google.com/search?source=univ&tbm=isch&q=How+I+can+quantify+H%26E+histology+image+for+tissue+using+Fiji?&sa=X&ved=2ahUKEwiZm9maw9bpAhWFM-wKHV4FBUUQsAR6BAgIEAE
You can use QuPath as a free open source whole slide image analysis platform.
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