Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Agarose gel purification pre MinION sequencing?

Dear all, we performed an agarose gel purification with MinElute kit (QIAGEN) to extract bands between 3500 bp and 6000 bp for further sequencing with MinION. After the purification process,...

16 February 2021 5,178 1 View

Does anyone have ever found a spontaneous expression of mast cells in Sash mice?

Hi all, I am working with Sash mice and in one of my experiments I saw two Sash mice that was double stained to cKIT and FCeRI. I was wondering if during time the cKit mutation can be lost? Does...

20 September 2020 6,876 2 View

How come the isotype signal is lower than the basal signal when analyzing flow cytometry?

Hi everyone I have performed several flow cytometry experiments and I keep getting a basal FITC signal (of unstained cells) higher than FITC isotype control signal (of cells stained with the...

26 July 2020 8,439 3 View

How can I concentrate peptides?

I am working with an initial volume of 20 mL* and using a 10 kDa filter to separate proteins from peptides. With this filter I am able to concentrate the proteins upstream of the membrane....

15 January 2020 1,520 7 View

Why is one plasmid not transfecting HEK 293t cells , while a simliar second plasmid sucessfully transfects the cells with the same protocol?

I ordered and produced a plasmid from Addgene containing PIK3CA and GFP (pHAGE-PIK3CA-C420R (item #116457). I performed mini- and midi preparations and verified plamid production with a...

02 October 2019 4,078 2 View

How do I quantify capsular polysaccharide in bacteria?

Hi. Is there a reliable protocol / method / kit for quantification of capsular polysaccharide in Acinetobacter baumannii or any other bacteria for that matter? preferably without the use of...

25 September 2019 7,303 3 View

No title

25 June 2019 498 2 View

Immunofluorescence - can I use a conjugated antibody and a primary not-conjugated different antibody - both from the same host species?

Has anyone ever tried to stain 2 different proteins in a single sample, while they both originate from he same species (1 of them conjugated though)? I have 1 primary antibody (host: mouse)...

06 May 2019 6,551 6 View

Does anybody know the name of this plant from the Canary Islands?

15 April 2019 1,244 6 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

What is recommended for solid domain in ANSYS FLUENT?

Should I mesh the solid domain ? or should I use wall thickness? or Shell conduction? for Heat Transfer between two fluids of a Heat Exchanger. what is recommended?

01 March 2021 9,785 3 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

How can i resolve the "final solutuin is not converged" error in Comsol for my heat transfer simulation??

I have been trying to simulate heat transfer problem in Comsol 5.5 for long time but it always throw an error stating: "Failed to find a solution. Maximum number of Newton iterations...

28 February 2021 8,626 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

How could I take into consideration the weights of observation in a system of seemingly unrelated regressions (SUR) in rstudio?

I build a model of 4 regression equations (probit and OLS) in R studio. I need to take the weights of observations into consideration. While it is simple to do so for every regression equation,...

27 February 2021 1,233 1 View

Can random sampling have inclusion criteria?

As random sampling means that everyone in the population have an equal chance to be chosen, can it have inclusion criteria? My research participants have been chosen based on the inclusion...

26 February 2021 6,905 10 View