Can any body advice if there is a protocol for calculating sample size from one group of sheep?

can any body advice if there is a protocol for calculating sample size from one group of sheep, population of 100 for instance? if there any software can i apply to get the minimum and maximum...

09 October 2016 6,500 6 View

Can anybody suggest the best way of increasing the specificity of Ryan 2013 nasted primers for 18S rRNA of Cryptosporidium spp detection?

can anybody suggest the best way of increasing the specificity of Ryan 2013 nasted primers for 18S rRNA of Cryptosporidium spp detection? in other word, that primer cross reacting with other...

05 June 2016 3,937 1 View

Can anybody suggest a useful tool for designing qPCR primers?

I am wondering if I could find reliable software of designing primers for qPCR

03 April 2016 6,316 5 View

Has anaybody tried to subject Cryptosporidium oocysts to FTA matrix?

I am wondering if anybody used FTA cards for Cryptosporidium DNA extraction?

01 February 2016 7,248 1 View

Cryptosporidium DNA works first time after extraction but did not work for next time?

10 November 2015 6,344 5 View

Has anyone tried using FTA cards for cryptosporidium DNA extraction? Is it possible subjecting fecal samples to FTA cards in other words?

Have anyone tried using FTA cards for Cryptosporidium DNA extraction? in other words, is it possible subjecting fecal samples to FTA cards? i hope to find answer to this question since my research...

03 April 2015 7,263 3 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Can anyone help with Schistosoma identification?

Can anyone identify what kind of Schistosome species this might be? Morphological features of cercariae and adults resemble Trichobilharzia, though I am fairly new to the field of parasitology...

25 February 2021 7,760 6 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View