DOes anyone have a good tissue dissociation protocol for FACs?

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Is there a problem with my RNA pellet?

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Saleh Alkarim

Hello

Look at links her may help you

Good Luck

http://jcb.rupress.org/content/199/2/225.full

http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0067801

http://www.tandfonline.com/doi/full/10.4161/cc.10.22.18150#.VtjHKH0rLIU

Hamid Bekri

Look at this paper ....

http://www.frontiersin.org/SearchData.aspx?sq=Transcriptomic+Analysis+of+Purified+Embryonic+Neural+Stem+Cells+from+Zebrafish+Embryos+Reveals+Signaling+Pathways+Involved+in+Glycine-Dependent+Neurogenesis&ht=1

good luck