I am staining mouse skin cryosections (10um) with PanH3, an antibody that binds to histone 3. When harvesting this tissue I embed in OCT and freeze using dry ice and liquid nitrogen. When viewing under a a fluorescent microscope, these sections appear to be significantly distorted and damaged even when no AGR (0.5% SDS) or fixation (0.5% PFA) is applied. The epidermis looks completely garbled and the dermis contains webby, hazy areas. I have applied DAPI to a few slides w/o going through my staining protocol and they seem fine so I am sure my sectioning technique is ok. Can anyone explain what I'm seeing (image attached)?