Samples were formalin fixed or 4% PFA fixed. Decalified in EDTA 14% for 2-3 weeks. I see that the TRAP has been used by some. But does anyone know if the ALP works too? Or any protocol to see ALP in paraffin sections ?
I didn't get ALP to work at all. I had some staining with TRAP but I definitely had to incubate a lot longer than the instructions indicated to see any results. Have you tried either kit yet?