Hi y'all,
does anyone know if animal cells increase in size as they age or do they simply multiply?
I usually do a Percoll gradient centrifugation to isolate endothelial cells but with pups it doesn't seem to work.
As far as I'm aware these gradients depend on cell size and if the cells were a lot smaller/larger that would explain my difficulties.
Thank you in advace!!
Hello Bree Barand
Yes, you are right. Animal cells increase in size as they age. Cells experience changes with aging. As cells near senescence, proliferation slows, but cell growth or the addition of cell mass continues relatively unchecked. The end result is a gradual increase in cell size with age. Old mammalian cells are often two or three times larger than young cells.
Attached below are a list of references that may help.
Article The relationship between cell size, the activity of DNA Poly...
https://pubmed.ncbi.nlm.nih.gov/6021926/
Article Effect of donors age on growth kinetics of rabbit articular ...
Article Evolution of the distribution, proliferation and ultraviolet...
Best.
- Badges
- Science topic
- Similar topics
- Analytical Chemistry
- Extraction