What underlying physiological mechanisms contribute to the drought avoidance observed in early flowering genotypes?

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How can I regenerate a SCX column modified with Sulfonate group (Thermo 054995)? The colmun has lost its Resolution and peak to valley ratio?

The column is used in Ion Exchange Chromatography of Monoclonal antibodies and the method of analysis is based on pH gradint.

13 July 2024 7,462 1 View

Does sucrose interfere with the BCA reagent in peptide concentration assays?

Hello everyone! I am currently working on the expression and purification of an antimicrobial peptide that experiences reduced activity after each freeze-thaw cycle. To improve its stability, I...

10 July 2024 6,830 1 View

What is the reason of stacking DNA in agarose gel well?

we electrophoresis PCR product on 0.7% agarose gel at 120V for 1 hour, our target size is 5000 bp but our DNA stack at wells and do not com in to gelldo you have any suggestion for this?

02 July 2024 5,235 0 View

Is it possible to increase the intensity of UV-vis absorption of DNA EITH A LIGAND IN the groove BINDING mode?

Is it possible to increase the intensity of UV-vis absorption in the interaction of the groove connection mode?

29 June 2024 4,867 4 View

Hello, I wanted to know if it is a necessity to perform MTT test in three time points to evaluate the cytotoxicity of a metal alloy?

I am working on a magnesium based alloy, and i wanted to evaluate the cytotoxicity of my alloy with MTT assay. I want to know the reason why most of the researchers perform the MTT assay in three...

28 June 2024 2,570 1 View

Population coverage analysis for some MHC II alleles?

Hello everyone Does anyone know how could we determine population coverage of some MHC II alleles such as HLA-DRB3*02:02, HLA-DRB5*0101, HLA-DPA1*02:01-DPB1*05:01, HLA-DRB4*01:01,...

20 June 2024 2,155 1 View

Separating Wind Wave and Swell Parameters in Wavewatch III Model?

Hello, I am using the Wavewatch III model for my research . Could anyone clarify if it's possible to extract wind wave and swell parameters separately from this model?

12 June 2024 3,269 4 View

Depletion igg from serum?

Hi everyone, I wanna get a suitable protocol for depletion of IgG and other immunoglobulins from serum, without kits or chromatography but with chemical methods like ammonium sulfate. Can anyone...

10 June 2024 9,523 2 View

How many sample of serum need to validate proteomics data ?

Hi everyone, I wanna do proteomics of monkeys serums in 2 states, healthy and patient. How many monkeys in each group I need to run proteomics and validate my data from it? Do I deplete albumin...

04 June 2024 4,091 3 View

How do I western blot of serum proteins without any unspecific binding of secondary antibody to my blot?

Hi everyone, I have some important problems with my western blot against serum proteins. I have used secondary antibody conjugated by HRP that binds in unspecific manner to other proteins as same...

03 June 2024 5,993 8 View

Request for Advice: Starch Metabolism Research Project?

I am currently considering a research project focusing on a comparative analysis of starch metabolism in orchids and roses. I am particularly interested in identifying the types and quantities of...

30 July 2024 4,267 2 View

China (Qinghai) flowers identification?

Can someone help me in identification of some flowers observed in Tibetan plateau (Qinghai province)? See the attached pdf. Sincerely Armando

18 July 2024 5,125 4 View

Hello all Can we use different concentrations of lemon or orange extract to grow flowers in ornamental trees and shrubs?

I need more pdf on this topic

02 July 2024 6,988 1 View

How do bees work on glassbrush flowers?

I need pdf information to increase my knowledge of this pollinator, which is honeybees

27 June 2024 3,815 1 View

How do i get bias corrected climatic data using python?

Hello, scientific community hope you are doing well. I am modeling the prediction of meteorological drought using the climate data (RCP4.5 & 8.5). However, I got stuck in the bias correction...

18 June 2024 4,667 3 View

Hello all I am currently working in extracting plant parts using ultrasound. Can I use the GC-MS technology or chromatographic analysis?

A number of active compounds and their concentrations found in flower and leaf extracts were obtained using this technique

09 June 2024 1,872 4 View

How does tillage contribute to climate change does drought increase adoption of conservation tillage practices?

02 June 2024 622 3 View

Is the liquid inside the flowers of the flower brush shrub mixed with honey?

I asked this question because I noticed bees sucking nectar during the day, and when examining flowers, the floral nectar increases at night.

01 June 2024 9,228 3 View

Does anyone share his experience regarding the Autophagy activity in plants under drought stress stress?

Please share your ideas and suggestions regarding that "Exogenous application of melatonin reduces autophagy activity in plants under drought stress conditions". If someone knows the phenomenon...

20 May 2024 6,561 1 View

How can we Map availability and quality of Water using satellite images using AI?

Satellite images are available for various bandwidths. How can we reap benefits of AI to classify and recognise the status of water bodies? How can we predict the availability of quality water for...

16 May 2024 3,523 2 View