1. Is algae culture environment requirement different from typical PTC?
2. As per my reading of some of the papers, in case of freshwater algae, it is recommended to use glutaraldehyde, instead of formaldehyde and Lugol's solution. If I preserve my freshwater algae sample with glutaraldehyde, can this sample be used for algal culture afterwards, suppose within a span of 1-2 months?
Glutaraldehyde, formaldehyde and Lugol will kill the algae cells. Algae can be preserved in 5% methanol at -80^C. See link for further details: https://utex.freshdesk.com/support/solutions/folders/19000160658
1. you can use typical plant tissue culture room set up for culturing your freshwater algae but be sure to use algal culture Media.
2. you can't use both the chemicals to save your freshwater algae, because both the chemicals are used to kill and preserve the algae to study its morphology alone... after killing you can't culture... so collect fresh and culture directly... if you need any trying on algal culture kindly contact www.aaribioscience.com
With Regards,
Nithya.
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