In Science Magazine this week, X. Xie wrote a short piece, on how the MM equation holds at the single-molecule level:
⇢ http://bernstein.harvard.edu/papers/Science-2013-Xie-1457-9.pdf
One of the references led me to the paper below, also from Xie's lab, which I don't fully understand:
⇢ http://bernstein.harvard.edu/papers/weimin_jpcb_2006.pdf
The relevant part of this seems to be:
“We assume that, for a given conformation, the binding kinetics can be described by the usual rate equations of chemical kinetics (i.e., the substrate concentration is low enough that the translational diffusion does not significantly influence the time course of the kinetics) and that the binding of substrate is pseudo-first-order with the substrate concentration ([S]) being time independent.”
I say that's the relevant part, though that's kind of what I'm asking: where in the paper does it "prove" that MM holds at single-molecule level?
The Science paper says:
“In single-molecule turnover experiments, each enzyme experiences constant substrate and product concentrations in the volume of interest”
Can anyone shed some light?
http://bernstein.harvard.edu/papers/weimin_jpcb_2006.pdf
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