Could someone tell me how to adjust the pH of Arginine to 9.8? If possible please suggest some papers on arginase assay with crude cell lysates after some drug treatments.
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
I'm a chemistry student and I'm researching the kinetic degradation of mixed plastic waste. I'm having trouble calculating the activation energy by the coats & Redfern method. The data that...
24 February 2021 974 1 View
Hello, I am an undergraduate student doing my dissertation, where I isolate PBMCs from leucocyte cones in order to plate monocytes and allow for macrophage differentiation. Although the numbers...
09 February 2021 5,334 8 View
Hello, I'm trying to identify good putative macrophage markers for flow cytometry in rats.
04 February 2021 3,913 4 View
I culture RAW 264.7 cells on my samples and differentiate them to osteoclasts with RANKL. I want to do resorption pit assay by culturing cells for 28 days. My problem is that the macrophages keep...
01 February 2021 6,746 2 View
Hi, I'm a student and I'm doing an internship. I need to asses the M1 and M2 populations of a culture using flow cytometry. From my researches, I know that this is subject to some controversy, as...
26 January 2021 299 3 View
Currently, I am working with RAW BLUE cell lines to check the SEAP expression on addition of TLR ligands? Method developed for different TLR ligands. How experiments can design for validations?...
24 January 2021 589 2 View
I am wondering if anyone has had success with in vitro polarization (via cytokines) of Jurkat cells. My goal is to understand the affect of a drug on Treg induction and differentiation. I...
12 January 2021 3,606 2 View
Hello, I will start an experiment using macrophage (THP-1 treated with PMA). I have ordered the plate that suitable for fluorescence plate reader which is glass 24 wells black uncoated tissue...
05 January 2021 4,214 3 View
I'm referring to activated carbon activation. Activation process does increase the surface area and functional groups content in activated carbon compared to biochar and raw biomass. But I'm...
30 December 2020 6,514 1 View