We estimate the FAD saturation of purified an acyl-CoA dehydrogenase homologue by the ratio of the absorption maxima in the range of 268nm, 366nm and 449nm (ratio of 5:0.7:1). We consider all FAD binding sites to be fully occupied.
Now we would like to compare this to other members of the acyl-CoA dehydrogenase.
Perhaps data contained in this book might be useful fro you:
Structure and Dynamics of Macromolecules: Absorption and fluorescence studies. -
isbn=0080474489 -
J.R. Albani - 2011 - Technology & Engineering
You could also check the book: Flavoprotein Protocols (Methods in Molecular Biology). It will tell you how to measure the FAD ratio in the protein and how to calculate the extinction coefficient.
Thank you very much. Of course we already now how to determine the amount of FAD. Nonetheless, we would like to compare the ratio of the absorption maxima with other acyl-CoA dehydrogenases.
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