Can you suggest me the best de -nova genome assembly tools ?

I have sequenced the complete genome of a plant species using two technologies The estimated genome size using flowcytometer is estimated to be 300Mb 1. Illumina(mate pair) 2. Oxford Nanopore (for...

11 December 2017 5,594 5 View

What is the protocol for Isoelectric focusing of a Brain sample?

02 March 2016 6,458 3 View

I want to make silver nano particles from pomegranate peel for the first time in our lab can all of you suggest how to go about it?

Protocols for the production of silver nano particles from Pomegranate peel

11 December 2015 7,411 3 View

In cytoscape when an network is constructed on basis of what is this network constructed how to i basically i analyze the network?

How to analyse a network constructed trough cytoscape

02 March 2015 660 4 View

Have any one used Gromacs for SIMULATION?

02 March 2015 2,469 4 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

SARS-CoV-2 Spike Protein Western Bands?

Hello, I am western blotting SARS-CoV-2 spike protein from lentiviral using an antibody against S1. To prepare samples, I mixed concentrated lentivirus 1:1 with 2X Laemmli buffer and boiled...

07 February 2021 3,643 4 View

Difference between assessing EC50 in presence of physiological levels of human serum albumin and alpha1 acid glycoprotein vs. % human serum?

01 February 2021 1,825 1 View

How would you assess protein binding adjusted EC50 for a drug that has a binding rate of over 95%?

I'm in need of a reference or a method to come up with a protocol that allows one to get the protein-adjusted EC50 for an antiviral drug in presence of human serum albumin and acid glycoprotein.

27 January 2021 1,402 1 View

How to calculate enzyme's energy of activation without rate constant (+excel)?

I want to calculate an enzyme's energy of activation, but I don't have the enzyme's molecular weight to calculate the rate constant or the pre-exponential factor A. This is part of an assignement...

25 December 2020 8,738 3 View

What is apparent Km Value of an Enzyme, Is it different from usual Km value and Why it is important?

It is very much confusing when some papers use this term "apparent Km of an enzyme even if they use single substrate ? Not at all clear ! Please help me understanding this. Thanks in advance.

27 September 2020 3,522 7 View

What is the difference between PCR and the initial steps (before SDS gel) of DNA sequencing? Which method is more effective?

I apologize if my question is not clear. I have a pure chemistry background and I am very new in a Protein Chemistry and Enzymology science.

25 September 2020 7,968 5 View

Spike glycoprotein expression will be more in cho or hek 293 cell line ?

Spike glycoprotein will express in which cell line ?

22 September 2020 3,131 4 View

Why do ELISA tests for SARS-CoV-2 target the antibody instead of the antigen?

I'm wondering why existing ELISA tests for SARS-CoV-2 only target the immune system's antibody against the virus instead of the antigen - (S) spike glycoprotein? Then the assay could detect the...

29 March 2020 3,242 9 View

How do I Glycosylate a Protein?

I am in my first year of a biology degree and i'm struggling with some coursework. How would i go about setting up an expression system to create a glycoprotein. I understand how to over express a...

19 March 2020 6,605 4 View