The protein of interest is a glycoprotein and the 3D structure is not resolved yet.
I have sequenced the complete genome of a plant species using two technologies The estimated genome size using flowcytometer is estimated to be 300Mb 1. Illumina(mate pair) 2. Oxford Nanopore (for...
11 December 2017 5,703 5 View
Protocol for IEF of rat Brain sample.
02 March 2016 6,550 3 View
Protocols for the production of silver nano particles from Pomegranate peel
11 December 2015 7,507 3 View
How to analyse a network constructed trough cytoscape
02 March 2015 757 4 View
Gromacs for molecular dynamic simulation since i am fresher in using it i would request anybody who used it to guide me in using the same
02 March 2015 2,588 4 View
As I remember according the classical enzymology there are four types of inhibition: Competitive, Non-competitive, Uncompetitive and allosteric Inhibition . There is an enzyme with Ca2+ as a...
21 March 2024 7,334 4 View
I am using lectin-based ELISAs to probe the glycans of different glycoproteins. I had successful data that showed clear differences in the lectin binding profiles/patterns. However, I wanted to...
08 November 2023 1,632 0 View
Since proteins give positive test for biuret I was wondering could that be the case with glycoprotein? Also do glycoproteins have any general test like biuret?
27 October 2023 8,894 1 View
I want to stain muscle cells for presence of glycoproteins. For this I am trying to do lectin staining. However, right now I do not have a working protocol and I have come across 2 lectins in...
05 October 2023 3,898 2 View
Has anyone ever tried to culture Jurkat cells in serum free media (+/- additional growth factors)? I'm looking for a way to reduce the amount of glycoproteins/lipids for prolonged culture. Best...
19 June 2023 457 6 View
there are many types of carbohydrates(glycoprotein ) :neutral and acidic glycoprotein
30 April 2023 7,460 2 View
i am new to enzymology and want to study the discipline through history based books (i,e, how was the Kreb's cycle discovered, methods used then, etc). what are your suggestions to achieve...
07 April 2023 544 2 View
Hello everyone, I would like to block some specific glycan (Gal & GalNAc) on the surface of the cells to investigate their physiological roles, in particular for cell adhesion and cell-cell...
12 February 2023 5,540 0 View
Glomalin is a glycoprotein produced abundantly on hyphae and spores of arbuscular mycorrhizal (AM) fungi in soil and in roots. The glomalin test measures the content of this protein produced by...
21 January 2023 3,824 5 View
I am trying to simulate a glycoprotein and generated GROMACS input files using CHARMM-GUI Input Generator (Glycan Reader). My molecule is large and I want to use a 4fs time step for the...
30 November 2022 3,513 0 View