I want to measure MnP activity by using the methodology described by Kuwahara and I need the molar decadic absorption coefficient for phenol red.
How can i find this coefficient?
Be careful with the pH. The absorption is greatly dependent on the pH.
Then, look at these spectra:
http://www.google.es/imgres?imgurl=http://www.phys.rush.edu/ERios/meas_0image7.gif&imgrefurl=http://www.phys.rush.edu/ERios/measuring_intracellular.htm&h=234&w=336&sz=6&tbnid=2lw-zzNKrcF7GM:&tbnh=90&tbnw=129&zoom=1&usg=__NlgUXSrNs2qYjWxNyaM0Gbo3dHs=&docid=3D0DrIW9pov9pM&sa=X&ei=pqilUs74Ju2q0AWT3oGIDA&ved=0CEMQ9QEwAw
When the temperature reaches 200 ° C, the solution turns brown but does not solve the isophthalic acid.
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