Is there any specific synthetic substrate for amylase of T.castaneum?

More Neeta sudamrao Khillare's questions See All

How can I reduce the reaction time of amylase with 4-nitrophenol alpha d maltopentaoside to form yellow color?

I have extracted, purified and lyophilized protein of insect.I use BES, HEPES and phosphate buffer.The yellow colour product is forming but after 24hrs.The reaction is carry out at 25-28 degree...

08 September 2016 1,712 7 View

How can I prepare BES buffer for amylase assay with varying pH?

please help me to prepare BES buffer for amylase assay.i have 4-nitrophenol alpha d maltopentaoside (synthetic substrate of amylase).

11 December 2015 3,309 3 View

Is there any specific method for preparation of crystals of amylase?

I have purified amylase which was extracted from insect sample.

04 May 2014 824 3 View

Is there any specific method to study the autocatalysis of enzyme?

During storage of crude extracted enzyme I observed that the number of bands are varying during gel electrophoresis.

08 September 2013 6,161 7 View

If we will increase the percentage of ammonium persulphate for polymerization of NATIVE PAGE, is there any effect on % of gel?

I already used 10% APS but there is a problem for polymerazation so instead of 10% I used 50%APS.

05 June 2013 575 8 View

What is a method to purify extracted alpha amylase from biological material?

Amylase was extracted by the centrifugation method, i.e crude amylase.

04 May 2013 9,151 2 View

Is their any easy method to identify male and female insect of Tribolium castaneum?

I have mixed culture collected from stores.

04 May 2013 9,350 5 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Do you consider undergoing a structure literature review / Meta analysis and getting numerical findings sort of quantitative method?

I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .

22 February 2021 1,131 4 View

Can I do two kinds of mixed method in explanatory research?

Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...

20 February 2021 6,897 1 View

Any Homemade Organic Synthesis Practices?

Hi everyone, I am a teacher of Heterocycles Synthesis, Is there a home practice that you recommend for undergraduate students? A simulator would also be useful Due to the pandemic, students...

18 February 2021 2,769 4 View

How to analyse a (perceptual) study using differing/comparable sets of stimuli?

I plan on running an online perceptual experiment that has, let's say, 100 unique video stimuli altogether. Since these are too many to be rated by every participants, I would like to present the...

15 February 2021 7,444 2 View

How to analyse a split-plot design in stata ?

I have run an split-plot design experiment evaluating the efficacy of a treatment (with 3 whole plots and 4 sub-plots), and I am planning to analyse the collected data in Stata. However, I do...

14 February 2021 7,800 7 View

Can I use a likert survey for criterion sampling in a qualitative study or would that make it mixed methods?

I am doing a phenomenographic study on the reading experiences of avid v. Neutral v. Non-Readers. If I use a likert survey to criterion sample for participants, does that make it a mixed method...

09 February 2021 8,594 3 View

How to create the context-mechanism-outcome configuration (CMOc) in the realist methodology?

In the realist methodology (especially by Raymond Pawson et al), the CMOc is a common structure to explain what works, for whom, in what circumstances, and why? I'm always asked about the...

08 February 2021 2,792 1 View

Safri Ishmayana

There are some published methods to synthesize amylase substrate. You can check these papers:

1. Farkas et al. (1997) Carb. Res. 303: 407-415 (http://www.sciencedirect.com/science/article/pii/S0008621597001870)

2. Usui et al. (1988) J. Biochem. 103: 969-972 (http://jb.oxfordjournals.org/content/103/6/969.short)

3. Damager et al. (2004) Carb. Res. 339: 1727–1737 (http://www.sciencedirect.com/science/article/pii/S0008621504002320

Hope this help..

Cheers

Marcia Moss

You can buy an amylase substrate from standard companies.

Ellis C O'Neill

Essentially you measure the increase i reducing ends released from amylose. Check the sigma website for a protocol.

http://www.sigmaaldrich.com/technical-documents/protocols/biology/enzymatic-assay-of-a-amylase.html

Robert Rej

I am unfamiliar with the amylase from T.castaneum, however, a convenient (commercially available) substrate for measuring human amylases is ethylidene-blocked 4-nitrophenylmaltoheptaoside (EPS-G7). A full method was published by Klaus Lorentz in 2000:

http://www.clinchem.org/content/46/5/644.full.pdf

Neeta sudamrao Khillare

Thank u very much for your answers.