Is there an easy method for liposome preparation?

I'm trying to use new organic compounds as a remedy for sperm membrane injury, I want to use lysosomes as delivering vehicles? can any one give me an easy method for liposome preparation?

03 April 2018 1,462 3 View

What is the best method to dissolve DPPH in the aqueous systems ?

I'm working on the use of DPPH in the biological system, I need o dissolve DPPH in buffer system , but DPPH is soluble in methanol and not soluble in aqueous solutions . how can I dissolve it.

01 February 2018 336 0 View

Why the absorbency in catalase assay (Aebi method) is unstable?

I use Aebi method in the assay of catalase, but i have a problem : the absorbency is unstable which mean that the numbers increase and decrease rapidly.

10 November 2015 2,533 34 View

Is there an rapid and easy staining procedure for determining sperm morphology?

09 October 2015 2,844 5 View

How I can induce male infertility in mice?

08 September 2015 7,898 14 View

Any one have a free program for IC50 determination in DPPH antioxidant assay?

08 September 2015 3,740 2 View

How do I perform derivative spectroscopy in the assay of lipid conjugated dienes?

I need to assay lipid conjugated dienes in food sample using UV derivative spectroscopy? I have no any information about how to do it?

03 April 2015 4,344 2 View

What is the extraction method for determination of MDA in a meat sample?

See above

08 September 2014 5,872 9 View

In Aebi method for CCATALASE assay.. how I can convert K to International Unit of enzyme

I used Aebi method foe CATALASE assay but the equation result is reaction rate (K), how I can convert it to international unit.

08 September 2014 5,758 0 View

Do you know a method for cyclooxygenase inhibition assay?

Assay of COX inhibition ability of plant extracts.

05 June 2014 10,039 0 View

How to calculate enzyme's energy of activation without rate constant (+excel)?

I want to calculate an enzyme's energy of activation, but I don't have the enzyme's molecular weight to calculate the rate constant or the pre-exponential factor A. This is part of an assignement...

25 December 2020 8,738 3 View

What is apparent Km Value of an Enzyme, Is it different from usual Km value and Why it is important?

It is very much confusing when some papers use this term "apparent Km of an enzyme even if they use single substrate ? Not at all clear ! Please help me understanding this. Thanks in advance.

27 September 2020 3,522 7 View

What is the difference between PCR and the initial steps (before SDS gel) of DNA sequencing? Which method is more effective?

I apologize if my question is not clear. I have a pure chemistry background and I am very new in a Protein Chemistry and Enzymology science.

25 September 2020 7,968 5 View

How to isolate/quantify a fungal enzyme for comparison of control vs. treatment?

I want to compare the amount of an enzyme's expression (laccase for example) or presence in the tissue as a result of the experimental treatment. Is there a protein similar to an antibody that...

31 December 2019 1,359 2 View

How strong is Flag tag binding to Anti-Flag antibody?

I am trying to plan an pulldown assay to find some prey protein for my bait protein. I am interested in using the flag tag because it is small and doesn't disturb structure and doesn't require...

11 December 2019 2,406 3 View

How to calculate the dosage of enzymes in wheat flour?

I have recently started working on improvement of wheat flour for baking. I have come across a research which involved the usage of xylanase, cellulase, beta-glucanase and carbohydrases for...

10 November 2019 3,608 1 View

Can I restrict the amount of one species when using KinTek for kinetic fitting?

I am using KinTek software to do a kinetic fitting. I have determined kinetics of the formations of several species in the time course of 5min and established a model for the enzyme (just as an...

09 October 2019 3,835 4 View

I am searching the pGex or pET plazid of HDAC6, if he is possibly for somebody and would give for me. Does somebody have this plasmid?

I am searching the pGex or pET plazid of HDAC6, if he is possibly for somebody and would give for me, would be needed for my work. I would like to test the interaction between my labor's new...

05 June 2019 4,133 1 View

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08 January 2019 3,512 5 View

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31 December 2018 8,083 5 View