in the estimation of Catalase the method assay K value, what is the equation to convert it ti IU.
I'm trying to use new organic compounds as a remedy for sperm membrane injury, I want to use lysosomes as delivering vehicles? can any one give me an easy method for liposome preparation?
03 April 2018 1,567 3 View
I'm working on the use of DPPH in the biological system, I need o dissolve DPPH in buffer system , but DPPH is soluble in methanol and not soluble in aqueous solutions . how can I dissolve it.
01 February 2018 481 0 View
I use Aebi method in the assay of catalase, but i have a problem : the absorbency is unstable which mean that the numbers increase and decrease rapidly.
10 November 2015 2,902 34 View
I want an rapid and easy staining method for determining sperm morphology.
09 October 2015 2,961 5 View
I want to do my experiment o male infertility via inducing it in mice, what is the chemical method for inducing infertility.
08 September 2015 8,009 14 View
I need a free program or a method for determination of IC50 in DPPH method of antioxidant assay.
08 September 2015 3,897 2 View
I need to assay lipid conjugated dienes in food sample using UV derivative spectroscopy? I have no any information about how to do it?
03 April 2015 4,445 2 View
See above
08 September 2014 5,995 9 View
I used Aebi method foe CATALASE assay but the equation result is reaction rate (K), how I can convert it to international unit.
08 September 2014 5,854 0 View
Assay of COX inhibition ability of plant extracts.
05 June 2014 10,200 0 View
As I remember according the classical enzymology there are four types of inhibition: Competitive, Non-competitive, Uncompetitive and allosteric Inhibition . There is an enzyme with Ca2+ as a...
21 March 2024 7,334 4 View
i am new to enzymology and want to study the discipline through history based books (i,e, how was the Kreb's cycle discovered, methods used then, etc). what are your suggestions to achieve...
07 April 2023 544 2 View
I want to calculate an enzyme's energy of activation, but I don't have the enzyme's molecular weight to calculate the rate constant or the pre-exponential factor A. This is part of an assignement...
25 December 2020 9,030 3 View
It is very much confusing when some papers use this term "apparent Km of an enzyme even if they use single substrate ? Not at all clear ! Please help me understanding this. Thanks in advance.
27 September 2020 3,825 7 View
I apologize if my question is not clear. I have a pure chemistry background and I am very new in a Protein Chemistry and Enzymology science.
25 September 2020 8,275 5 View
I want to compare the amount of an enzyme's expression (laccase for example) or presence in the tissue as a result of the experimental treatment. Is there a protein similar to an antibody that...
31 December 2019 1,557 2 View
I am trying to plan an pulldown assay to find some prey protein for my bait protein. I am interested in using the flag tag because it is small and doesn't disturb structure and doesn't require...
11 December 2019 2,620 3 View
I have recently started working on improvement of wheat flour for baking. I have come across a research which involved the usage of xylanase, cellulase, beta-glucanase and carbohydrases for...
10 November 2019 3,870 1 View
I am using KinTek software to do a kinetic fitting. I have determined kinetics of the formations of several species in the time course of 5min and established a model for the enzyme (just as an...
09 October 2019 4,046 4 View
I am searching the pGex or pET plazid of HDAC6, if he is possibly for somebody and would give for me, would be needed for my work. I would like to test the interaction between my labor's new...
05 June 2019 4,351 1 View