How does one quantify total cellulose, hemicellulose, and lignin in biomass before pre-treatment of lignocellulosic biomass?
Lignocellulose is a renewable biomass which is widely available in nature. It contains cellulose (glucose), hemicellulose (xylose, arabinose, mannose etc.) and lignin. How one can analyse a perticular lignocellulosic (wheatbran) biomass to quantify the above to detect the quantity of each of them before and after pretreatment of lignocellulosic biomass. Is there any specific and simple methods are available to analyse. please let me know.
Thank you
There are simple volumetric methods of analysis. You refer any of the Biochemistry lab manual e.g. Laboratory Biochemistry by Ramanna
TAPPI method for determination of cellulose, hemicellulose & lignin in lignocellulosic biomass.
NREL laboratory analytical procedures (LAPs):
http://www.nrel.gov/biomass/analytical_procedures.html
Try this.
Relux the biomass sample at 80 C with 0.5 M NaOH for 4 h, (100 ml/ g of biomass) filter and wash the residue with plentiful of distillled water until PH 7- this should give you hemicellulose.
Soak another sample with at room temperature in concentrated H2SO4 (30ml/g of biomass) for 24 h, and then reflux at 100 C for 1h. Filter and wash the residue with distilled water until PH-7 - this should give lignin.
You can deduce cellulose by difference.
We use Ankom Fiber Analyzer 2000 to do these tests in our lab. You can look for the equipment on their website: Ankom.com.
Good luck
I want to suggest NREL laboratory analytical procedures, attached is one of the procedure. It is easy and straight forward.
I agree with the indication of Mr. Pandey.
Although there are several methods, I use the TAPPI method in my research and I have achieved great results.
Yes, the NREL procedures have a broad use but you have to evaluate the facilities that you have in your lab.... then to know the basis Tappi procedures to adjust some parameters..
alternatively you may use Van Soest Methods for ADF and NDF and from that you can do lignin, cellulose, hemicellulose and ash. JUst type in google you will get it
Hi
may be test this method
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0110000
is it possible to perform an accurate determination of glucose and cellulose without the use of an HPLC ?
Veronica, glucose can be determined using a NH2 column.
See this paper.
Glucose and other neutral monosaccharides in lignocellulosics can be determined also by gas chromatography (see attachment), however derivatization is required.
- Badges
- Science topic
- Similar topics
- Biological Science
- Biochemistry
- Enzymology