I am looking for an enzyme that can hydrolyse a fucoidan polysaccharide into oligosaccharide units.
i am also looking for such an commercially available enzyme that degrade fucoidan.
Is it possible to determine the antibacterial minimum inhibitory concentration (MIC) using a liquid inhibition assay (MIC) with Lysogeny broth medium instead of Mueller-Hinton broth? If so, is...
01 February 2020 7,312 5 View
I am searching for a supplier to purchase the iCHIP or a similar device for in situ cultivation of sedimentary bacteria. I am looking at different technologies to try to culture previously...
10 November 2018 9,430 0 View
I am conducting a comparative study trying to isolate bacteria on different concentrations of Marine broth (MB) media + agar vs MB + gellan gum/Gelzan/Gelrite. For the agar I have 10, 50, and...
10 November 2018 7,427 4 View
I am trying to extract community DNA from deep-sea sediments using MP BIOMEDICALS FastDNA™ SPIN Kit for Soil. I have used starting material from 50 mg (recommended amount for marine sediments) to...
10 November 2017 5,047 3 View
Can fastidious or even unculturable marine bacteria be grown in situ? If so, what are the current methodologies for doing so?
03 April 2017 1,578 2 View
I am looking for standard nutrient solid media recipes or protocols used to culture marine bacteria. What are the standard types of media used to culture marine bacteria (psychrophiles)? Also, in...
03 April 2017 9,654 4 View
Is it expected that MCF12A cells should show some level of cytotoxicity to anticancer compounds or drugs? If so, what could be the biochemical or physiological reason therefore?
11 December 2015 7,372 4 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I'm very interested in biotechnology and medical research and I'd like to meet a professional here and talk about it together
28 July 2024 3,798 4 View
List down the various factors responsible and how does this effect the overall biogas production when it is related to Hydraulic retention time of the digester based on the feedstock?
27 July 2024 4,800 4 View
Dear Siesta Users, I installed Siesta 5.0.1 and I want to use Grimme's D3 correction. According to the 5.0.1 manual, I could use a few parameters to include the D3 correction in the...
27 July 2024 5,748 0 View
It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...
25 July 2024 8,738 0 View
PDE value for Tert-Butylamine
24 July 2024 1,166 0 View
I run mechanochemical reactions that involves the use of a large amount of catalyst (1:1 molar ratio to the reactant). When I try to understand the effect of amount of catalyst on the reaction, I...
23 July 2024 9,938 1 View
I am pleased to announce the CUSABIO Biotechnology Scholarships for Students 2024! This scholarship aims to support outstanding students pursuing studies in the field of biotechnology. Here are...
22 July 2024 5,790 1 View
Hi, I am a PhD student working at Jagiellonian University, Faculty of Chemistry. My work is related to the development of new analytical methods for the quantification of short-chain fatty acids...
22 July 2024 5,492 6 View