Which is the best concentration of DEPC in water solution to inactivate larger amounts of RNases?
In what solution do you need to inactivate larger amounts of RNases and why such amounts of RNases where? As a rule, 0.1 % (v/v) DEPC-treated-autoclaved water is used for preparing water RNase-free solutions. But. DEPC reacts with A’s and to some extent G’s resulting in the loss of the base and spontaneous strand breakage of the RNA backbone. So, you should eliminate all traces of DEPC in the water by autoclaving. We have never used DEPC-treated water. If you have good water (eg milli-Q) it’s not necessary to treat by DEPC.
In what solution do you need to inactivate larger amounts of RNases and why such amounts of RNases where? As a rule, 0.1 % (v/v) DEPC-treated-autoclaved water is used for preparing water RNase-free solutions. But. DEPC reacts with A’s and to some extent G’s resulting in the loss of the base and spontaneous strand breakage of the RNA backbone. So, you should eliminate all traces of DEPC in the water by autoclaving. We have never used DEPC-treated water. If you have good water (eg milli-Q) it’s not necessary to treat by DEPC.
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