Why in biolarvasida experiments we have to use 3rd and 4th instar larvae of mosquito ?

Give me reason of that, thank you.

29 December 2018 5,908 6 View

Is there any calibration of Acoustic velocity from MFA?

16 October 2016 2,972 4 View

What causes the formation of the tail and the head of the compound in a thin layer chromatography ?

Thin layer Chromatography

08 March 2016 2,866 4 View

Which one of SDS-PAGE and Native-PAGE i should choose to analyze enzyme related resistance of insect?

Dear all, I want to analyze enzymes related resistance of insect (i.e. Carboxylesterase, Acetylcholinesterase, Esterase, Glutathione S-transferase, Mixed Function Oxidase) by electrophoresis, but...

02 March 2016 8,308 3 View

Can we use DMSO instead of betaine?

18 January 2016 682 3 View

How to do time scale in CFD simulation?

If we want to simulate long period case, we must use time scale to reduce our simulation time. But, I still dont understand the effect of NS Equation. How is the effects?

12 September 2012 8,431 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

Why is there cloudy white solution in flavonoid test tube?

I do flavonoid test for biscuit incorporated with muntingia fruit powder. Sample extracted with metanol (1:10 ,w/v), macerated 24h, sentrifuged and filtered. Supernatan used for analysis. I have...

08 February 2021 6,779 4 View

No title

Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...

08 February 2021 7,285 1 View

How define one baker unit(BU) is the amount of hydrogen peroxide catalase ?

07 February 2021 2,006 1 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View

What are the conflicts between EIA and environmental engineers and lawyers?

Environmental Impact Assessment (EIA) does not live up to the expectation of environmental lawyers and environmental engineers. In your own opinion, how can the EIA process therefore be made more...

03 February 2021 1,329 6 View

What is the maximum storage time for samples used in soil enzyme assays?

01 February 2021 4,616 3 View