Despite single-cell RNA, how can I characterize a new cell subtype/phenotype?

A new cell subtype was found out only during regeneration phases after a cuprizone-induced demyelination model. How can I characterize this cell? It is important to highlight that was already...

13 February 2021 1,245 1 View

How can I improve RNA extration from human neutrophils?

I tried several times extrating RNA from human neutrophils and the amount of RNA at the end of the precedure was minimal. I also tried using colunns from QIAGEN. Never had any problem with tissue...

26 January 2021 8,881 4 View

A light for the oxford nanopore rapid attachment chemistry. Does anyone know how this works?

Some Oxford Nanopore kits have Primers with 5 ' modification that provide ligation to adapters without the need for ligases (ligase-free method), as in 16S barcoding kits. Does anyone know what...

21 December 2020 8,612 3 View

How can I merge Scopus and WOS metadata?

I am conducting a systematic review and need to merge the metadata of the articles resulting from the search in these databases (Web of Science and Scopus) to analyze in WOSviewer. How can I do this?

17 December 2020 9,292 3 View

Calculation the Pump power needed in a pressurized flow system?

Hi all, I am design now a liquid high pressure flow system to be used up to 50 bar, and I am having some doubts concerning how I can estimate the pump power. Besides the pressure drop in the...

15 December 2020 8,411 1 View

Design of a membrane system to remove CO2 from a flue gas current?

Hi all, I am trying to design a membrane system to remove the CO2 from a flue gas current, and I am having difficulties in finding information. Does anyone have any technical information,...

15 December 2020 3,655 2 View

When available, are you planning to receive SARS-CoV-2/COVID-19 immunization? What do you most fear about it?

If possible, let us know about the which vaccine would be available in your geographic location.

06 December 2020 3,527 23 View

Can the potential of LIPIDMICS improve the diagnosis and understanding of metabolic changes in various neurodegenerative disease (PD, HD, AD, MS, etc?

08 November 2020 7,209 1 View

What pressures should I choose in a reheated rankine cycle?

09 October 2020 9,117 4 View

Can we use efficiently capillary zone electrophoresis (CZE or FSCE) to separate nanoparticles?

Can we use capillary zone electrophoresis (CZE or FSCE) to separate nanoparticles, please? Are there works in the literature on this topic? What are the main difficulties to develop...

04 September 2020 3,385 5 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View