I'm trying to standardize a buffer in which my biotin conjugated and HRP conjugated molecules (antibodies and antigen) stay stable for longer time.
My assay for detection of HIV works well with no background. The only issue is the stability of my conjugated molecules. In accelerated stability studies my current conjugate diluent gives more than 50% reduction in activity at 9th day at 37 degree Celsius.
So, can anyone suggest me a solution or if any buffer recipe?
Thanks in advance.
Swapnil , you can use commercial avaiable stabilizers https://shop.surmodics.com/protein-conjugate-stabilizers-learn
I tested SZ-02(for HRP-conjugates) and SZ-01 (for Biotinconjugates)
Thank you Antonina Kokisheva for the help. I have used surmodics buffers and it does helps to some extent. Also, I'm trying to make the assay cost effective and so avoiding the commercially available buffers.
Thanks again.
Use of 20 % glycerol or 1% BSA (protease free, fraction V) can improve storage stability of antibodies and other proteins.
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