Hi everyone,

I am trying to detect Amyloid oligomers in brain tissue extract of APP mice by ELISA kit but the outcome is mostly undetectable.

I am using IBL-Elisa kit: Amyloid Beta (82E1-specific) Aβ Oligomers (ref27725) and I have problems detecting it. I homogenize 5-10mg of tissue in Tris buffer pH7.4 (20mM Tris; 140mM NaCl) with a pestle (not sonication).

I have searched for publications about this form of samples processing but I don’t get any clear suggestion about it.

Anyone knows if it is necessary some other protocol or previous-step for low-weight samples?

Any advice are welcome.

Thanks in advance!

Ángel

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