Hi everyone,
I am trying to detect Amyloid oligomers in brain tissue extract of APP mice by ELISA kit but the outcome is mostly undetectable.
I am using IBL-Elisa kit: Amyloid Beta (82E1-specific) Aβ Oligomers (ref27725) and I have problems detecting it. I homogenize 5-10mg of tissue in Tris buffer pH7.4 (20mM Tris; 140mM NaCl) with a pestle (not sonication).
I have searched for publications about this form of samples processing but I don’t get any clear suggestion about it.
Anyone knows if it is necessary some other protocol or previous-step for low-weight samples?
Any advice are welcome.
Thanks in advance!
Ángel