I won´t do an ELISA assay but I'll do a biochemical assays, like lipase activity assay, lipid peroxidation or total carbohydrate assay. Can I use the ELISA reader without filters? What is the function of filter? Thanks!!!
Hello, Diana. The filters allow the you to select that the range of the visible spectrum that will be passed through your sample. Without the filters in place, you would simply be passing all of the light through. Our plate reader will allow us to operate it without a filter. Obviously, the only concern is that you don't have any resolution/ability to distinguish different colored species. But, if you only have one colored species present, then there shouldn't be any significant issues. Although this limits resolution, it ought to actually improve your sensitivity.
Best regards,
-Daniel King
What sort of plate reader have you been using? If it is a filter-based instrument, it probably already has filters built in, or it might use a monochromator to select the wavelength instead of filters.
Hello, actually filter is a glass which setting your wavelength, actually if you see filter option in equipment it means no monochromator there. With filters you have from 6 to 8 combination of wavelength. For lipase assay use filter 450nm.
Lambert-Beer's law is valid only for monochromatic light. Remember also, that in a photometer you measure the difference between incoming and outgoing light, from the point of error propagation, subtraction is the worst possible operation. If the amount of light is increased by irrelevant wavelength, that would become worse. So without filter, you'd get a non-linear absorbance vs concentration curve and a higher standard deviation of your measured values.
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