Hi all,

I recently did a project where I did carboxyl-to-amine immobilisation with a monoclonal interferon gamma antibody (human ELISA development kit, Mabtech). I had carboxyl groups on a carbon surface which I activated using EDC/NHS. This was successful, but I am unsure about the location of the amine groups on my antibodies.

Most antibody driven immobilisation documents give an image of an antibody with a carboxyl group located at the end of the Fc region. This obviously cannot be the location of the "linker" as I am looking for amine groups on my antibodies.

Would you be able to help me get a source that could give me the relevant information regarding the above-mentioned interaction or a more detailed explanation of the antibodies that I used.

Kind regards,

Stephan

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