Hi there!
We are applying L.lactis for expression of a heterogeneous antigen using a cell wall anchor subunit. Cloning process has been verified and expression optimization is up now but I'm getting confused with the whole cell ELISA result. It seems control population have different properties at the surface such as, more tight attachment to the wells and agglutination through mouse antiserum treatment despite of test population. Can any one help me out to find an explanation for what I have observed repeatedly?
Any idea is welcomed
Thanks a lot
Things I can think of.
Cell membrane properties are highly dependent on the phospholipid membrane component, sub-membrane attached proteins, and aggregations caused by interaction of islands such as pores, or creating of leaking/channels.
Does the antigen binding change the membrane potential, for instance?
Does the antigen binding change the membrane constituents?
Does the antigen binding change normal cell function?
We did x-ray diffraction on RBC ghost membranes with contrast such as Uranyl ions. Seems to change the membrane average thickness, I would suspect thru propogation of electrostatic charge. Retinal cells fire with the binding of a single photon amplified.
Dear Dr. Bilash ;
Your idea is possible theoretically but actually I m looking for someone who has the same experience for bacteria after displaying of a protein at the surface.
Thanks for sharing your thoughts.
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