Hi,
I was thinking if it is possible to do a immunoassay using a partial peptide (the same I used to produce the antibody for that same immunoassay) as a standard? Also would it be useful in a competition ELISA? Does anyone know of a protocol for this?
Thanks,
Diogo
This polypeptide can be used in immunoassays. As a neutralizing antibody, it is good to verify the antibody. However, this assay has the disadvantage that it does not verify the selectivity of the antibody for the antigen, since the non-specific binding activity of the antibody will also be inhibited by the blocking peptide. Thus, blocking peptides can prove that antibodies are bad, but they do not prove that antibodies must be good.
Could you describe your ELISAs? And how long is the peptide? Its difficult to give an opinion without more details.
Gertrudis Rojas It is a 203 aa hormone in sea lamprey. The immunoassay is a competition style.
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