De-embedding of OCT kidneys?

08 August 2019 1 5K Report

Hi,

I have snap-frozen (isopentan-bath) mouse kidneys stored at -80°C and embedded in OCT. How can I de-embedd them to use for a Nephrin ELISA?

Thanks.

Valeria Tananska

Hi,

Have a read:

Article An Optimized Method for Protein Extraction from OCT-Embedded...

Badges
Science method

More Konstantin Gradel's questions See All

Snap-freezing in isopentane bath with(out) OCT?

Hello there, I want to snap-freeze mouse kidneys for immunofluorescence using isopentane surrounded by liquid nitrogen (1/4 of kidney for 1 min and then rapidly in -80°C freezer). Is there...

26 July 2019 5,165 2 View

Snap-freezing in isopentane bath with(out) OCT?

Hello there, I want to snap-freeze mouse kidneys for immunofluorescence using isopentane surrounded by liquid nitrogen (1/4 of kidney for 1 min and then rapidly in -80°C freezer). Is there...

06 July 2019 9,838 2 View

What is the weight of rotavirus A (ng)?

I am developing an ELISA kit for rotavirus A (G1P8 and G1P6). Does anyone know the weight (ng) of these two strains? Or to help me find their weight?

29 July 2024 3,138 2 View

Ideal tissue thickness for multiple immunolabelling and confocal microscopy?

Hi there! I'm hoping to to look at co-localisation of cytokines and markers of different brain cell types (e.g., neurons, astrocytes, microglia) in juvenile rat brains (postnatal day 28) that have...

23 July 2024 2,844 1 View

ELISA sandwich with two monoclonal antibodies ?

hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...

16 July 2024 9,044 10 View

Daniel Everett versus Noam Chomsky on Language?

Many have criticized Noam Chomsky’s theory of language (e.g., Pinker as described in Sihombing 2022), but the most effective criticisms have come from Daniel Everett, given that Chomsky (according...

15 July 2024 492 4 View

What do I do with dried urine samples?

I placed mice in metabolic cages to collect urine samples for analysis. However, the majority of the samples have dried up. Is there any way to retrieve the urine in those tubes without...

14 July 2024 6,724 0 View

Why is my zebrafish sample falling out of the agarose embedding?

Hi all, I am looking for advice on what could be going wrong with my zebrafish caudal fin tissue fixation that would cause the sample to fall out of the embedding while cryosectioning. During cryo...

25 June 2024 4,602 0 View

How do I prepare rat cardiomyocyte cell lysate for ELISA via freeze-thaw method?

Anyone have a protocol for making cell lysate from rat cardiomyocyte pellets to run an ELISA using PBS as the buffer?

23 June 2024 2,264 4 View

What is considered a biological replicate when you are doing an ELISA plate measurement with different cell types?

If cells obtained from different passages, with different plating but harvested on the same day are used in a single plate to take an ELISA measurement (with the given standards and treated...

17 June 2024 6,705 1 View

Different interpolated concentrations?

I used two standards (one came with the ELISA kit and the other I made myself, by preparing a series of known concentrations of the target analyte) on the same plate in my ELISA run. Why are the...

15 June 2024 5,109 1 View

Question about ELISA with the "Cattle IGF 1 (Insulin like growth factor) ELISA Kit" Cat: ELK5630?

Dear fellow researchers, We are conducting an ELISA with the "Cattle IGF 1 (Insulin-like growth factor) ELISA Kit" (Cat: ELK5630). Samples and reagents were prepared following the instruction...

13 June 2024 9,473 0 View