17 October 2020 1 693 Report

Dear Colleagues

I am interested in evaluating the concentration of SERUM succinate dehydrogenase in a certain type of cancer. I found an ELISA kit for it however, I can barely find any papers measuring it in serum, but rather mostly in tissues.

With my research, tissue sample from patients are not feasible, only blood. Is there any problem if I assayed it in blood? Can anyone help me in this point please?

TIA

More Yasmine Amr Issa's questions See All
What can explain a low expected heterozygosity rate for T. monococcum wild samples (0.09) ?

I conducted an SDS PAGE electrophoresis on wheat wild varieties to separate their glutenins and measure the genetic diversity of my sample. I only ran one electrophoresis. Can a genetic drift...

14 February 2021 3,971 1 View

What is suitable temperature at reaction mixture of lipase activity assay?

I use para nitrophenyl palmitate substrate with tris buffer ph 8... i read many temperature for Yellow color of para nitro phenol production.. 37 and 50 and 40 at water bath ... I confused as i...

04 February 2021 9,100 5 View

What is the relationship that determines the depth of the mechanical cultivator with the age or size of the weed For crops planted in lines?

the great depth of the cultivator may lead to passage under the roots without killing them and may give negative results to break up the soil below the roots, increasing their activity, especially...

03 February 2021 3,198 7 View

Does the subsoil have an effect on the spread of weeds whose roots are more than 35 cm deep?

For weeds whose roots deepen more than the level of subsoil, they may be affected by this layer, for example, determined by height and growth, as well as limited to flower production and seeds.

01 February 2021 1,940 4 View

Is there any evidence that using ball milling in intervals, rather than one long run, improves the mixing process?

I am trying to synthesize nanoparticles by mixing the precursors by ball milling. I would like to know if mixing them for one hour straight is less or more efficient than mixing them in intervals...

10 November 2020 8,527 9 View

How to monitor and mapping crisis event tweets based on my tweets dataset?

how to monitor and mapping crisis event tweets based on my tweets dataset. I need tools for that. I was classified as tweets to damaged and not. then I have extracted locations from textual...

18 August 2020 4,068 2 View

Which unsupervised learning methods can be used to detect fake users?

In an online website, some users may create multiple fake users to promote (like/comment) on their own comments/posts. For example, in Instagram to make their comment be seen at the top of the...

12 August 2020 3,736 12 View

My melanoma cell line doesn't grow well suddenly, May i know the possible reasons for that?

I have 92-1 of melanoma cell line , when i thawed them they were totally normal , but after first passage , the cells begun not growing well and not totally attaching on the bottom of the flask...

30 June 2020 3,622 4 View

How to compare pre-trained models with traditional models in the field of NLP?

There are new models such as BERT that requires pre-training and fine-tuning. And there are the traditional models, such as DecisionTree and SVM, which requires us to extract features from the...

20 March 2020 7,784 2 View

Possible reason to change the color of carbon dots from greenish into blue after immobilization process?

Dear experts, Liquid carbon dots (CDs ) were synthesized with greenish color under UV-light. However, after mixing with PVA polymer, the color turned to blue with enhanced quantum yield (QY). what...

16 March 2020 5,304 4 View

Similar questions and discussions
How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close