Looking for good/reliable betaine assay kits. Does anyone have experience with such a thing?

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Why am I not able to use all VEP programs for the in silico analysis of avpr1b SNP's?

Good day researchers I am busy analysing the predicted effects of certain SNP's in AVPR1b. When using the VEP prediction programs (SIFT, Polyphen, FATHMM, LRT, Provean, Mutation Assessor, and...

28 February 2021 5,197 1 View

Should we reduce the standard unit of length to 0.31951540592 meter ?

The reasoning behind this question is to align the ratios of the proton potential in energy per charge to the speed of light in distance per time. As these two dimensions appear to be related,...

27 February 2021 947 7 View

What metal thin film houses also do annealing?

Could anyone provide names of metal thin film deposition vendors that also do high temperature annealing, in the range of 300-500 C?

27 December 2020 6,137 3 View

Vacuum freeze drying protocol in human skeletal muscle tissue methods, anyone?

I will be freeze-drying small skeletal muscle samples (~60mg) per sample using a Virtis SP Scientific Benchtop Pro Freeze Dryer. I've had difficulty findings a reference protocol to determine the...

21 November 2020 1,423 1 View

Does anyone freeze samples in GeneJET RNA extraction kit lysis buffer?

For the GeneJET RNA purification kit from Thermo Scientific, does anyone know if you can lyse your sample (suspension cells in my case) in the lysis buffer from the kit and then keep the sample at...

18 October 2020 4,993 3 View

Accurate curvature estimation of CT-scans (image stack)?

We have multiple CT-scans in the format of TIF image stacks. To get the mean curvature we first transform the stack to a triangular mesh (using Marching Cubes). To make the triangles in this mesh...

03 August 2020 7,921 4 View

Input from experts in environmental survey sampling design on draft sampling design decision tree?

Hi. I am trying to help provide some rough advice for selecting amongst alternative sampling designs to measure bird density or abundance. The surveys are short-term in duration such as for impact...

22 January 2020 2,978 3 View

Test for silicone residue from silicone part?

I have a silicone part that will come in contact with food. It will need to pass FDA tests for hexane extraction of silicone residues. Is there a method I can use to remove uncured material and...

09 December 2019 4,246 3 View

Use Betas Estimated in Original Jones Model in Modified Jones Model STATA?

Hello, I can use the following codes in Stata to generate regression according to original Jones model. gen Jones1991_DA=. forval y = 2010(1) 2015{ forval i = 1(1) 6{ display `i' display `y'...

14 October 2019 6,438 1 View

Difference between Resistive and Inductive Switching?

Both types of switching are used to characterise the transistor ON-time (tON), rise time (tr), fall time (tf), OFF-time (tOFF). What are the differences between the two characterisation methods...

26 August 2019 4,797 1 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View