I am using an organotypic hippocampal slice culture where we are exposing our slices to alcohol with subsequent alcohol withdrawal.
I want to make sure I am capturing the right time to assess TNFa concentrations. We are currently collecting and assessing 24 hours after the treatment, but I'm concerned that TNFa concentrations may be more transient (i.e. TNFa may act quickly and be subsequently degraded in a window of time that I'm missing at 24 hours compared to something like 30 minutes or an hour after treatment).
Does anyone have any suggestions/can anyone direct me to a reference regarding the optimal window of time for assessing TNFa concentrations?
ELISA details: analytical sensitivity of
You can check on 12 and 24h. How is it going your control? Are you using LPS?
The controls do not differ from the EtOH groups. We have not tried LPS yet, though this seems like the most appropriate next step.
Thanks!
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