hello , I ask for papers wich use acetone to dissolve plant extracts instead of DMSO
thank you
DMSO usually considered as inert suspending material used for dilution of insoluble ingredients
DMSO usually considered as inert suspending material used for dilution of insoluble ingredients
Hello Rajae Rajae,
Dissolving crude plant extract with DMSO is a typically done for antibacterial assay. It should noted that the concentration that normally permitted is aroun 2-5 % v/v. Using a pure DMSO could hinder the actual result.
Best Regards,
Zulfadhli.
Hello there Rajae.
Please keep in mind that using DMSO, like Zulfadhli mentioned, in high concentrations might hinder the results besides the fact that DMSO is extremely hard to remove from samples/extracts.
In any case, it depends on what you'll be doing with the extracts: will DMSO be an interferent?; will DMSO be necessary to be absent after extraction?.
Using pure acetone for plant extraction wouldn't be my first choice but water/acetone mixtures, EtOH or hot water are more common. You can also use EtOAc or Hx, depending - again - on the purpose of the plant extracts.
Articles using acetone and other solvents for plant extraction follow:
https://doi.org/10.1081/LCSS-200026796
https://onlinelibrary.wiley.com/doi/abs/10.1002/%28SICI%291099-1565%28199811/12%299%3A6%3C274%3A%3AAID-PCA416%3E3.0.CO%3B2-G
Article Comparison of Different Solvents for Phytochemical Extractio...
Article The effect of different solvents and number of extraction st...
https://hrcak.srce.hr/file/146876
Regards,
Filipe
Hello there Rajae.
Adding to my answers as previously stated a negative control should also be tested, i.e the 2-5% v/v DMSO solution only (without the extracts) to be sure that it does not affect the result you are obtaining. :D
Regards.
Zulfadhli
You're welcome!
If you can, please let us know which one did it worked for you as it might help other people with the same question.
Best of luck for you.
Filipe
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