Does freezing effect DMSO (For MTT) efficiency?

More Masoumeh Fardi's questions See All

How to find the best I-Gain & P-Gain parameters in AFM contact mode?

I am trying to do Roughness Measurement for Alumina templates . I am getting streaks when playing around with I-Gain & P-Gain but not a good image of publication quality. Could someone suggest...

30 May 2024 2,956 4 View

How to synthesis the uniform polyamide active layer on the aminated PVC support layer?

we work on forward osmosis membrane. the support layer is aminated PVC and the active layer is polyamide. for synthesis of polyamide , I follow the routine methods of interfacial polymerization...

19 April 2024 3,574 1 View

Introduction of an alloy with high temperature oxidation, HNo3 and HCl resistance?

Hello. Can you introduce a traditional alloy or HEA, with high temperature (1200 degree) Oxidation resistance and boiling HNo3 and HCl resistance?

13 November 2023 8,479 1 View

How to detect Asparaginase resistant cell lines?

Dear friends How to detect Asparaginase resistant cell lines? Do you know cell lines resistant to asparaginase? Is there any protocol to detect the resistance?

20 August 2023 7,261 1 View

Can siRNA be delivered into human normal/leukemia cell?

Hi Do you know that siRNA can be inserted into the white blood cells of normal people and leukemic people sample?

11 July 2023 7,580 1 View

Recombinant protein protocol?

Dear colleagues Please suggest a comprehensive and complete protocol for the production and purification of bacterial recombinant protein in E.Coli BL21 strain.

10 July 2023 8,318 2 View

How can I calculate C-statistic for specific time interval in STATA?

for example, how can I calculate c-index or c-statistic (for investigation of my prediction model performance) at 5-year and 10-year follow-up in cohort sample?

04 October 2022 6,666 2 View

Do you have a standard scfv production protocol?

I want to make scfv from E.coli, but I can't find a standard and clear protocol. Please help me if you can🙏

20 September 2022 2,378 1 View

What type of phosphate buffer is suitable for extracting protein from the plant?

What type of phosphate buffer is suitable for extracting protein from the plant I intend to extract and measure protein from plants by using the Bradford method. In order to do this, I need a...

13 March 2022 2,771 6 View

Is it possible that XRD peaks sharpen with prolonging the milling time?

Hello. My XRD data shows that with prolonging the milling time, peaks become more sharp and have higher intensity. Is there an explanation for it?

08 February 2022 743 7 View

Can formaldehyd-fixed gelatin be melt?

I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...

07 August 2024 1,686 1 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

Melt curve problem in qPCR?

Do these peaks in the curve indicate primer dimer or non-specific products?

01 August 2024 7,466 0 View

What is the best protocol for freezing the rat brain in preparation for protein detection by Elisa ?

What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?

01 August 2024 1,722 1 View

I need to dissolve stigmasterol for an animal experiment, it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent?

Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?

01 August 2024 7,504 2 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

What Are the Best Alternatives to the MTT Assay for Assessing Cell Toxicity of Colored Herbal Extracts?

I am currently investigating the cytotoxicity of a series of herbal extracts, and like many studies, I have been using the MTT assay to evaluate cell viability. However, I am encountering a...

31 July 2024 193 4 View

Matthew Carson

DMSO freezes easily (freezing point of 19 degrees) so this is not uncommon. I also froze DMSO accidentally, but used it without issue. The freezing should not effect its efficiency - it quite a stable compound.

Masoumeh Fardi

Thank you...I was so nervous about this.

Sharafaldin Al-Musawi

Hi dear Masoumeh Fardi

I think the following papers can help you;

Best and kind regards.

Article The Effect of Freeze/Thaw Cycles on the Stability of Compounds in DMSO

Article Effects of freezing rates and dimethyl sulfoxide concentrati...

generally it doesnt effect on DMSO efficiency