Does freezing effect DMSO (For MTT) efficiency?

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Matthew Carson

DMSO freezes easily (freezing point of 19 degrees) so this is not uncommon. I also froze DMSO accidentally, but used it without issue. The freezing should not effect its efficiency - it quite a stable compound.

Masoumeh Fardi

Thank you...I was so nervous about this.

Sharafaldin Al-Musawi

Hi dear Masoumeh Fardi

I think the following papers can help you;

Best and kind regards.

Article The Effect of Freeze/Thaw Cycles on the Stability of Compounds in DMSO

Article Effects of freezing rates and dimethyl sulfoxide concentrati...

generally it doesnt effect on DMSO efficiency