Do I use ethanol acetone to fix and permeabilize cells for transwell migration assays

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Jayant Dewangan

Acetone is not the good choice to fix cells in plastic cell culture plates or transwell. Insted of that i use 100% methanol. It works fine.

Steingrimur Stefansson

Hi Chamath, I agree with Jayant. Stay away from acetone because it dissolves plastics.

As for the concentration of Matrigel, I used 1 ug/ml in PBS to coat the transwells to look at cell migration.