Does anyone get a high background fluorescence when imaging cells stained with CellRox Red? Any suggestions on how to reduce?

31 January 2017 1 6K Report

I am staining primary macrophages in complete media. There is a high background when imaging the media controls as well as the positive control (400uM TBHP)

Any suggestion on how to reduce? Thank you!

Amira M Gamal-Eldeen

what is your fluorescence microscope brand? Is it Zeiss?

The microscope regularly possessed a control of the fluorescence gain and of the laser exposure intensity.

You should decrease the fluorescence gain and decrease the exposure intensity too.

In more advanced microscope you can also decrease the red color curve to have a clear black background.

I hope it helps.

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