What is the best voltage to electroporate CRISPR RNP complex into Kasumi 1 cells?

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Are there SDMs that can take in percentage data?

Hi everyone, I am wondering if anyone knows of a species distribution model that can take in percentage data (i.e. presence data with prevalence values attached to them)? The dataset I have...

18 February 2021 7,312 3 View

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When I research the ransomware detection with adversarial examples, I extracted the dataset from the strings based on cuckoo dynamic analysis. Those datasets are based on the count of each string....

08 February 2021 7,049 3 View

Do you have any suggestions for me to modify my LS-DYNA model?

Hi, I use LS-DYNA to simulate the steel composite shear wall now. I use MAT_084/085 to simulate concrete, MAT_159 to simulate steel plates, and MAT_024 to simulate studs. The concrete section...

24 January 2021 3,863 1 View

Corpus linguistics collocate indicative of diversity?

I have a question, as to whether collocations in corpus linguistics can be used to indicate diversity. I have a corpora of media news articles, and I manage to find out the frequency of my target...

19 January 2021 1,446 1 View

2SLS vs mediation?

I have been trying to understand 2 stage least squares regression, and I am having difficulty understanding how that is different from mediation analyses. It would be helpful if you could provide...

08 January 2021 7,848 3 View

What is a good reference on designing experiments to determine molecular mechanism of disease or biological process?

I need to learn techniques and strategies in planning experiments that try to figure out the molecular mechanisms. Thanks.

01 January 2021 2,525 3 View

What is the significance of Per-Patient and Per-Tooth analysis in epidemiology?

Research in prevalence usually provide results in per-tooth/object level and per-patient level. What is the significance of these two values and which is actually more important/accurate? What are...

10 December 2020 4,248 4 View

For SIADH, does it cause edema and Hypertension?

I have found two teachings from different sources. One they said about blood volume increase and therefore the patient has oedema and hypertension. But, another source says when there is high...

08 December 2020 1,278 3 View

What is the white layer on my fermented natto bean paste?

Dear all, Good day! Recently i tried to ferment my blended soya bean powder mixed with Natto bacteria culture in a Natto maker (containing some water) at around 45 degree celsius overnight....

23 November 2020 7,105 20 View

Why do I keep getting smudged and wavy bands in certain lanes of my western blots?

Hi everyone, Been doing western blots for years now, recently however, whenever I run 2 gels, one of the gels would have a few lanes that exhibit wavy or smudgy bands at around 50 kDa (see...

16 November 2020 3,759 11 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Is there a classification scheme for rock cavities or caverns with respect to their size and shape?

The range of forms and sizes of cavities in rock is very big. Is there a classification scheme for rock cavities regarding their size and shape?

02 March 2021 4,511 3 View

How do I determine the degree of polymerization of PEG 400 based on NMR integration?

Hello, I am working on the photocatalytic degradation of PEG 400. I would like to know if it's possible to determine the degree on polymerization based on NMR integration, or by the MW of the...

02 March 2021 6,186 3 View

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

Can we distinguish the type of free fluid inside the pelvic and abdominal cavities whether blood or serous fluid using the ultrasound?

during ultrasound examination of acute abdomen, sometimes we see free fluid , can we know the type of fluid ,whether blood or not , using ultrasound only? if yes , what are the sonographic...

01 March 2021 7,476 4 View

Which is suitable for use with Python? MySQL or SQL Server?

Which is suitable for use with Python? MySQL or SQL Server? What is your suggestion?

01 March 2021 3,422 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View