Although there are several papers regarding EPS measurement in sludge, it is quite confusing. Like exactly what need to be measured , and how is that calculated. I would appreciate if someone could guide me for EPS measurement in water.
There are different methods for extracting and measuring EPS, depending on the type of sample and the purpose of the analysis. Generally, EPS can be divided into two categories: loosely bound (LB) and tightly bound (TB) EPS. LB-EPS is the EPS that can be easily removed from the cell surface by mild physical or chemical treatments, such as centrifugation, washing, or a low concentration of cation exchange resin (CER). TB-EPS are the EPS that are firmly attached to the cell surface or embedded in the matrix and require more intensive treatments, such as high concentrations of CER, sonication, heating, or steaming.
The most common method for measuring EPS is to first extract them from the sample using a suitable method and then quantify the main components of EPS, such as proteins, carbohydrates, humic substances, and DNA, using colorimetric or spectrophotometric methods. For example, the Lowry method can be used to measure proteins, the phenol-sulfuric acid method can be used to measure carbohydrates, the UV-vis method can be used to measure humic substances, and the Hoechst 33258 method can be used to measure DNA. The total EPS content can be calculated by adding up the contents of the individual components.
However, there is no standard or universally accepted method for measuring EPS, and different methods may have different advantages and disadvantages, such as extraction efficiency, reproducibility, accuracy, and interference. Therefore, it is important to choose a suitable method based on the characteristics of the sample, the research objectives, and the available equipment and reagents.
Shuaipeng Tian Thank you for suggestion but at least you could have suggested me some good papers that are worth to read in this topic.
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