Which stata commands can i use to generate the relative index of inequality?

I am using a relative index of inequality to assess health inequalities yin unmet need in family planning. Whereas I understand the steps to calculate this I am finding trouble with...

25 March 2020 4,206 3 View

Why does the slope looks like in vii7 (realtime)?

The test is for detecting the direct qualitative for Chlamydia trachomatis and Neisseria gonorrhoea. We use FAM (CT), JOE (NG) reporter and CY5 (beta for control). Results are reportable after we...

07 June 2017 9,398 2 View

Any idea where I could get Nicotiana tabacum cv. BY-2 seeds?

Thank you

30 November 2016 8,889 3 View

How can one determine the variance that arose in a study?

04 May 2016 5,570 2 View

When comparing two methods, is it adequate to use ANOVA or do I need to assess agreement as suggested by Bland and Altman?

I am comparing two methods of determining the strain of the heart. I have used one way ANOVA, but looking at past literature that compared methods of determining strain, they use Bland Altman...

02 May 2016 3,413 3 View

Can someone suggest me a good basic learning material for Dynamic Mechanical Analysis done on polymer composites

28 March 2016 8,094 8 View

How do one teach someone to understand the global and local alignments to a person who is new to bioinformatics?

How can one understand the sequence alignment in a more practical way based on the local and global alignments.

11 February 2016 3,141 3 View

What to do with G09 when displacement is high whereas force is low and predicted change in energy low?

Dear All, I optimize a structure with M06 / 6-311+g(d,p). After a lot of optimisation step , the predicted energy is low -1.7 D-07 and forces very low. However the calculation do not converge as...

28 October 2015 2,592 6 View

Can someone help with SDS PAGE- troubleshooting?

I was doing SDS-PAGE when i ran the dye started to seperate into two layers. i dont know why. the pH of the buffer and volt everything were ok. i checked everything buffer, pH, and also made the...

05 May 2015 7,505 3 View

Voltage fluctuating during transfer

Yesterday I did a western blot. During the transfer, i fixed the volt to 100 but it kept on fluctuating netween 90-80. What may be the reason?

04 September 2012 220 1 View

How can I assign a velocity along one direction to CO2 molecules in a molecular dynamics simulation using GROMACS?

Dear all, I am trying to perform a MD simulation about the interaction between CO2 and a polymer. I create my model, solvate it and perform the NVT and NPT equilibration. Now I am not able to...

26 February 2021 10,014 3 View

Recalcitrant seed short-mid storage method?

Dear peer scientists, Ultradry storage is not suitable for recalcitrant seeds. However, theoretically, if we can stabilize the structure of the subcellular membrane, as well as glassify the...

25 February 2021 2,442 1 View

Is there a suitable way to confine cell attachment area inside a multi-well plate?

Are there any types of cylindrical cell culture inserts that are designed to confine cell seeding area? Say, if I'm using a 12 well plate (3.5 cm^2/well), but only want to seed cells in the middle...

22 February 2021 7,127 4 View

Is it possible to use maize mature seed to do transformation for maize transgenic study?

Almost all research reports which i ever seen work on Early mature embryo for Agrobacterium mediated maize transformation. In other grass families like rice, we can use mature seed and what do...

17 February 2021 1,434 13 View

Dealing with BMDM seeding and RNA extraction ?

biomedical master student here , i am dealing with mice BMDM and would like some help , i seed 0.5 x 10^6 cells/per well in a 6 well plate but when i extract the RNA i get a very low yeild , i...

15 February 2021 1,102 5 View

Is secondary metabolite production more at vegetative growth stage or during reproductive stage?

Hi, I am working on the effect of heavy metals on a medicinal plant by pot experimentation. Analyzing the effect of heavy metals on secondary metabolites is one of the parameters. I will be...

11 February 2021 6,683 6 View

Are the topics below viable research in plant biotechnology?

1. Chemically induced mutagenesis in seed and qPCR detection and amplification of desired trait. 2. Site-directed mutagenesis in qualitative traits of a multiline seed.

04 February 2021 9,069 3 View

Seeds harvest from white clover by selfing?

I tried to get seeds from white clover by self-fertilization but I could not get the seeds. As you know the white clover is generally fertilized by crossing. I wanna get the seeds by...

04 February 2021 9,152 4 View

What is the best method of preparing cell supernatant samples for a Luminex assay to detect cytokines ?

I am planning to run a Luminex assay using cell supernatant to assess cytokine secretion by tumor cells. I came across different methods in literature; some suggest seeding in complete media and...

03 February 2021 4,867 3 View

How do I measure Arabidopsis germination rate?

Hi there, I'm looking for a way to measure the Arabidopsis germination rate, like what media I should use to plant the seeds and what indicator I should see for germination. I'm planning to...

02 February 2021 4,785 3 View