Selecting particular columns from large datasets (csv) using R Studio?

So I have large number of csv files (100+) in a directory with same structure and column names. The data is in time series and there are approx 30 columns/file. However i only want to aggregate...

08 February 2021 245 5 View

How much pre-PCR dilution of mixed-template DNA extracted from pollen (honey) is appropriate for Metabarcoding?

I'm working on pollen DNA metabarcoding to identify the floral composition of honey. How much pre-PCR dilution of mixed-template DNA extracted from pollen (honey) is appropriate for the...

28 December 2020 9,132 1 View

How to add two vertical and parallel Fresnel reflection coefficients to retrieve overall reflection coefficient for a right hand circular wave?

I have a problem in which a right hand circular polarized wave (GPS wave) encounters an ideal smooth plane obliquely, and then is reflected off that. To solve this kind of problems, the wave is...

28 December 2020 2,271 3 View

Which is better to focus on teaching or research for a good academic life and Why?

Which is better to focus on teaching or research for For a good academic life and Why?

26 December 2020 5,737 9 View

Is ag paste suitable for back contact on silicon wafer? or i should deposit al on the back surface using pvd or plasma sputtering?

Fabricating silicon solar cell

22 December 2020 4,906 7 View

Which renewable source is the best ?

wind hydro solar etc.

21 December 2020 6,360 16 View

Why is light pure energy?

light is pure because the light is the simplest electromagnetic construct but the energy of any object is also of the same kind.

20 December 2020 207 4 View

How to handle Missing Values for Skipped Questions (Systematic Missing) in R ?

10 December 2020 435 4 View

How to dye DOPC liposome with Dio c18 fluorescent dye?

13 November 2020 9,244 3 View

What is the best industrial emulsifier to make silicon oil in water emulsion?

I need to make long-lasting stable emulsions of 6 wt. % percent silicon oil in DI water. The previousely made emulsions using Tween and Span Emulsifiers did not remain stable after a week.

31 October 2020 3,568 3 View

Is aligning mitochondrial and nuclear sequences inconsequential?

Probably a silly question but I am a complete novice in molecular biology. I have decided to attempt a phylogenetic analysis. I have managed to create a phylogenetic tree but want to confirm...

19 January 2021 5,895 2 View

Matrigel dissolves during paraformaldehyde fixation?

Hello, I am working on 3D culture of MDCK cysts. Whenever i try to fix the cells with PFA, the matrix depolymerizes. We thought it was something to do with the matrigel, so we tested different...

01 February 2020 5,875 2 View

How to induced toxoplasma cyst in vitro?

I am trying to induce toxoplasma cyst formation (both RH and ME49) strains by altering the pH to 8.1 (DMEM, 1%FCS, 1% pen-strep), at 37oC, without CO2. However, the host cells weren't healthy and...

10 November 2019 9,882 1 View

Anybody knows what is this mineral?

I would be very happy if somebody would be able to tell me what is the mineral in bright on the back-scattered SEM photographs? It is constituted by Fe, Cl, O and Zn (the rest of the elements are...

31 December 2018 8,931 0 View

35 year old women presented with central periumblical pain specially with each menstrual cycle.What is the provisional diagnosis and definitive R ?

She had 3 children and previous history of ceasearian section. on abdominal US there is left ovarian cyst what is the provisionsl diagnosis and definitive treatment?

11 December 2018 2,379 0 View

How to lyse the wall of Gardia cyst?

I am trying to extract Gardia DNA from stool. What I succeeded to get so far is in range of 1000 bp which is far to short than published. Any suggestions?

09 October 2018 5,636 1 View

Please suggest a good protein extraction method for Symbiodinium/Dinoflagellate to be used in Western Blot?

Dear All, I am having problems in extracting the transmembrane proteins from Symbiodinium, but no problems with cytoplasmic proteins. The extraction medium I am using consists of Tris, sodium...

08 September 2018 7,548 1 View

Could anyone help me identify the dinoflagellate species?

This species was observed at coastal waters of Kerala,India

08 September 2018 3,627 0 View

Can ELISA be used to measure cyst fluid tumor markers?

In reviewing methodology of a manuscript, I found out that CA125 levels in ovarian cyst fluids were measured using an ELISA kit. In the kit manual, it says that this kit is used for measuring...

08 August 2018 1,547 3 View

Can ELISA be used to measure cyst fluid tumor markers?

In reviewing methodology of a manuscript, I found out that CA125 levels in ovarian cyst fluids were measured using an ELISA kit. In the kit manual, it says that this kit is used for measuring...

07 August 2018 7,010 4 View