Although the malignant tumors at the initial stage from the stemness starts as a Monoclonal origin, upon the further proliferation, a secondary clonal population of cancer cells are generated. This further continues to go and change the clonality.
Hence, do the malignant tumors need to be addressed as a monoclonal or polyclonal?
http://onlinelibrary.wiley.com/doi/10.1002/path.2793/pdf
Some molecular techniques can be used to identify clonality for example in Multiple Myeloma Ig gene rearrangements is used as an indicator. If all cells in a colony have the same rearrangement pattern they should be grown clonally. I think this is important because CSCs should recapitulate the disease on their own, from one cell origin. If a tumor bulk is polyclonal, it could be a mixture of different cells divided a couple times and that does not mean these cells are totally clonogenic, in my opinion.
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